The stability investigation of compound Danshen injection (a traditional medicine) with a new highperformance liquid chromatography method
Yanqing Zhang2,3, Fengqin Bao2, Zhixia Zhao2, Xiuyu Sun2, Wuqin Qi2,3, Junbo Xie2,3★★ Corresponding author
- 1Department of Pharmaceutical Engineering, Tianjin University of Commerce, China.
- 2Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China.
CORRESPONDENCE
Junbo Xie
Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China.
Received: 09-11-2012; Revised: 03-12-2012.
Volume 9, Issue 36 · pp. 338–343 · PUBLISHED 7 September 2013 · DOI: 10.4103/0973-1296.117830
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Compound Danshen injection (CDSI, a traditional medicine) is an effective drug for the treatment of cardiovascular and cerebrovascular diseases. However, the research about its stability is absent. Objective: A new high-performance liquid chromatography method was developed to assay its main effective constituents, i.e., propanoid acid (PA), protocatechuic aldehyde (PHA), salvianolic acid B (SAB), salvianolic acid A (SAA), and rosmarinic acid (RA). Through the newly found method, the stability of CDSI was to be investigated. Materials and Methods: The analysis was performed by a reverse-phase gradient elution using an aqueous mobile phase (containing 0.1% acetic acid) modifi ed by acetonitrile, and detection was made simultaneously at 280 nm and 325 nm. The method was validated for accuracy, precision and limits of detection. The effects of some environmental storage conditions (light and temperature) on the stability of CDSI were investigated. Results: This method is precise, simple, and convenient. The result showed that illumination and temperature had an obvious effect on CDSI’s stability. SAA is the most unstable one among the fi ve components. In the condition of common light, it decomposed rapidly to almost 50% after only 4 h, and 100% after 8 h. PA, RA, and PHA might come from Danshen, was also the transformed products from other components in store process. Conclusion: The result indicated that the main active constituents in CDSI suffered from the illumination and temperature greatly. CDSI should be stored at low temperature and kept away from light.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Zhang, Y., Bao, F., Zhao, Z., Sun, X., Qi, W., & Xie, J. (2013). The stability investigation of compound Danshen injection (a traditional medicine) with a new highperformance liquid chromatography method. Pharmacognosy Magazine, 9(36), 338–343. https://doi.org/10.4103/0973-1296.117830