β-cyclodextrin assistant flavonoid glycosides enzymatic hydrolysis
Xin Jin1, Zhen-hai Zhang2, E. Sun2, Xiao-Bin Jia2★★ Corresponding author
- 1College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210 046, China.
- 2Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR, China.
CORRESPONDENCE
Xiao-Bin Jia
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, PR, China.
Received: 16-08-2012; Revised: 06-10-2012.
Volume 9, Issue 36s · pp. 11–18 · PUBLISHED 7 September 2013 · DOI: 10.4103/0973-1296.117851
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: The content of icaritin and genistein in herba is very low, preparation with relatively large quantities is an important issue for extensive pharmacological studies. Objective: This study focuses on preparing and enzymic hydrolysis of flavonoid glycosides /β-cyclodextrin inclusion complex to increase the hydrolysis rate. Materials and Methods: The physical property of newly prepared inclusion complex was tested by differential scanning calorimetry (DSC). The conditions of enzymatic hydrolysis were optimized for the bioconversion of flavonoid glycosides /β-cyclodextrin inclusion complex by mono-factor experimental design. The experiments are using the icariin and genistein as the model drugs. Results: The solubility of icariin and genistein were increased almost 17 times from 29.2 μg/ml to 513.5 μg/ml at 60˚C and 28 times from 7.78 μg/ml to 221.46 μg/ml at 50˚C, respectively, demonstrating that the inclusion complex could significantly increase the solubility of flavonoid glycosides. Under the optimal conditions, the reaction time of icariin and genistin decreased by 68% and 145%, when compared with that without β-CD inclusion. By using this enzymatic condition, 473 mg icaritin (with the purity of 99.34%) and 567 mg genistein(with the purity of 99.46%), which was finally determined by melt point, ESI-MS, UV, IR,1H NMR and 13C NMR, was obtained eventually by transforming the inclusion complex(contains 1.0 g substrates). Conclusion: This study can clearly indicate a new attempt to improve the speed of enzyme-hydrolysis of poorly water-soluble flavonoid glycosides and find a more superior condition which is used to prepare icaritin and genistein.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Jin, X., Zhang, Z., Sun, E., & Jia, X. (2013). β-cyclodextrin assistant flavonoid glycosides enzymatic hydrolysis. Pharmacognosy Magazine, 9(36s), 11–18. https://doi.org/10.4103/0973-1296.117851