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    Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18

    Yong Zhang1★, Kunpeng Xue2, Eugene Yuexing Zhao2, Yin Li2, Lixin Yao2, Xiaoyan Yang1, Xiaomei Xie1★ Corresponding author

    1. 1School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei, 230031, China.
    2. 2Welch Materials (Zhejiang), Inc., Jinhua 321000, P.R, China.

    CORRESPONDENCE

    Yong Zhang

    School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei, 230031, China.

    Received: 11-09-2012; Revised: 06-10-2012.

    Volume 9, Issue 36s · pp. 19–24 · PUBLISHED 7 September 2013 · DOI: 10.4103/0973-1296.117853

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The RP-HPLC resolution of two triterpenic acid isomers was unstable. Objective: To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC. Materials and Methods: The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolution effects of mobile phase composition and different column temperatures were systematically investigated by using Drylab® (Rheodyne LLC.) after evaluating chromatograms automatically. Results: The best available resolution of two bioactive isomers was achieved (r = 3.4) via using PAH (polycyclic aromatic hydrocarbons) polymeric C18 bonded phase column. The chromatographic system was applied to the quantification in ten Chinese medicinal plants and the validation was carried out and the precision (RSD ≤1.34%), the linearity (r ≥0.9998) and the recovery (range from 92.1% to 102.6%) were acceptable. Conclusion: It is clear that the method was simple, rapid and reliable for the quantification of two compounds in new HPLC method within PAH polymeric C18.

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      Zhang, Y., Xue, K., Zhao, E. Y., Li, Y., Yao, L., Yang, X., & Xie, X. (2013). Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18. Pharmacognosy Magazine, 9(36s), 19–24. https://doi.org/10.4103/0973-1296.117853