Biological Screening of Tri‑Jannarose as a Recipe from Thai Traditional Medicine
Ladachart Taepongsorat1, Ampa Konsue2★★ Corresponding author
- 1Biomedical Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham, THAILAND.
- 2Applied Thai Traditional Medicine, Thai Traditional Medicine Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham, THAILAND.
CORRESPONDENCE
Ampa Konsue
Applied Thai Traditional Medicine, Thai Traditional Medicine Research Unit, Faculty of Medicine, Mahasarakham University, Maha Sarakham, THAILAND.
Volume 11, Issue 2 · pp. 110–114 · PUBLISHED · DOI: 10.4103/pr.pr_178_18
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ABSTRACT
Context:Tri‑Jannarose (TJ) is a Thai traditional medicine recipe, the ingredients of which are betel palm seed (Areca catechu L.), Siamese neem tree root (Azadirachta indica A. Juss.), and heart‑leaved moonseed vines (Tinospora cordifolia [Thunb.] Miers). The equal mixture of three plants indicated to treatment of antipyretic, diuretic, expectorate, nourishment and appetizing.Aims:Phytochemical screening, antioxidant, and α‑glucosidase inhibitory activities of TJ using different extractions were evaluated. Materials and Methods:The three plants of recipe were extracted using different solvents such as aqueous extract (ATJ), 50% ethanolic extract (HETJ), and 95% ethanolic extract (ETJ). The phytochemical screening was determined by total phenolic compounds and total flavonoid contents. The anti-oxidation were tested using by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and 2,2 -azinobis- (3-ethylbenzothiazoline-6-sulphonate) (ABTS+) assay. The α‑glucosidase inhibitory activity was determined for glucose transferase mechanism. Results: Phytochemical screening found that this recipe had both phenolic and flavonoid substances. The ETJ (IC50 = 0.0463 ± 0.002) was exerted on antioxidation higher than HETJ and ATJ (IC50 = 0.0511 ± 0.000 and 0.1485 ± 0.005 mg/mL). Surprisingly, ABTS+ assay, ETJ (IC50 = 0.015 ± 0.000 mg/mL), and HETJ (IC50 = 0.022 ± 0.000 mg/mL) showed high effect on free radical scavenging activity than the standard controls, ascorbic acid (IC50 = 0.025 ± 0.001 mg/mL), and Trolox (IC50 = 0.032 ± 0.001 mg/mL). The α-glucosidase inhibitory activity found that all of the extract including ATJ (IC50 = 0.0127±0.02 mg/mL), ETJ (IC50 = 0.0154±0.01 mg/mL) and ETJ (IC50 = 0.0202±0.01 mg/mL) were more potent to inhibit α-glucosidase emzyme than acarbose (IC50 =0.745±0.026 mg/mL) as a positive control. Conclusion:The pharmaceutical preliminary scarring was confirmed to treatment on Thai traditional medicine. The recipe composed with phenolic compounds and flavonoids contents which chemical substance were more potent anti-oxidation, and bittersweet flavor was stronger to α‑glucosidase inhibitory activity.
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Taepongsorat, L., & Konsue, A. (). Biological Screening of Tri‑Jannarose as a Recipe from Thai Traditional Medicine. Pharmacognosy Research, 11(2), 110–114. https://doi.org/10.4103/pr.pr_178_18