Toxicological Study of the Effect in vivo and in vitro of Artemisia herba-alba Aqueous Extract in Rats
Ali Lahna1, Naima Benjelloun1, Nadia Seddik1, Mernissi Farida2, Abdallah Naya1, Mounia Oudghiri1★★ Corresponding author
- 1Department of Biology, Immunology and Biodiversity Laboratory, Faculty of Sciences Ain Chock, Casablanca, MOROCCO.
- 2Department of Anatomo-Pathology, Hospital University Center of IBN Rochd, Faculty of Medicine, Hassan II University of Casablanca, Casablanca, MOROCCO.
CORRESPONDENCE
Mounia Oudghiri
Department of Biology, Immunology and Biodiversity Laboratory, Faculty of Sciences Ain Chock, Casablanca, MOROCCO.
Received: 04-02-2020; Revised: 09-04-2020; Accepted: 10-06-2020.
Volume 12, Issue 3 · pp. 207–211 · PUBLISHED · DOI: 10.4103/pr.pr_4_20
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ABSTRACT
Background:Artemisia herba-alba(AHA) is largely used in folk medicine in different countries. However, rare studies provided toxicological evaluation regarding their safety on human health. Objective: This study investigated the safety of the standardized aqueous extract of AHA, like used by patients, to evaluate their toxicity in vivo andin vitro. Materials and Methods: For toxicological evaluation in vivowe used acute (during 14 days) and sub-acute oral gavages in Wistar rats (rats treated daily for 42 days at 1–5 g/kg bw) and the 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay was performed to determine the level of cell viability and the degree of cytotoxicity in vitro(0–30 μg/ml) on cultured spleen cells. Results: The LD50was up to 2 g/kg. Signs of mortality and toxicity were observed after single doses and no-observed-adverse-effect levels in the sub acute toxicity was up to 2 g/kg bw. Compared to the control, the treatment did not produce any statistically significant changes on alanine aminotransferase and aspartate aminotransferase serum titer. However, for creatinine and urea serum value, a significant increase (P < 0.05) was observed. The histological observations of liver and spleen tissues have shown well-preserved normal cells. Indeed for kidney tissues some artifacts of retraction and vascular congestion were noted for 3–5 g/kg doses after sub-chronic treatment. The addition of plant extracts to the spleen cells did not show any sign of toxicity for all doses tested. Conclusion:We conclude that AHA aqueous extract at the dosage up to 2g/kg bw will be toxic and can affect mainly the kidney tissues.
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Lahna, A., Benjelloun, N., Seddik, N., Farida, M., Naya, A., & Oudghiri, M. (). Toxicological Study of the Effect in vivo and in vitro of Artemisia herba-alba Aqueous Extract in Rats. Pharmacognosy Research, 12(3), 207–211. https://doi.org/10.4103/pr.pr_4_20