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    Article

    Effect of Seasonal Variation on Euphadienol Content in Euphorbia antiquorum L. and Euphorbia tirucalli L. using High‑Performance Thin‑Layer Chromatography Method

    Sudipta Roy1, Rabinarayan Acharya1★, VJ Shukla2, Anagha Ranade3★ Corresponding author

    1. 1Dravyaguna Department, IPGT and RA, Gujarat Ayurved University, Jamnagar, Gujarat, INDIA.
    2. 2Pharmaceutical Chemistry Laboratory, IPGT and RA, Gujarat Ayurved University, Jamnagar, Gujarat, INDIA.
    3. 3CCRAS Unit, RAIFR, Pune, Maharashtra, INDIA.

    CORRESPONDENCE

    Rabinarayan Acharya

    Dravyaguna Department, IPGT and RA, Gujarat Ayurved University, Jamnagar, Gujarat, INDIA.

    drrnacharya@gmail.com

    Received: 12-01-2020; Revised: 26-02-2020; Accepted: 29-04-2020.

    Volume 12, Issue 3 · pp. 236–242 · PUBLISHED 14 August 2020 · DOI: 10.4103/pr.pr_1_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Ayurveda emphasizes that different parts of plants (both organized and unorganized) should be collected on a certain season to get their better yield and therapeutic value. In general, Kshira (latex) should be collected in Sharada rutu (autumn season). However, the latex ofSnuhi has been stated to be collected at Shishiranta(end of winter). The latex ofSnuhi has an important place in different therapeutic formulations. One of the important mentions is for the preparation of Ksharasutra, one of the widely used paramedical procedures in Ayurveda. Objectives:The objective of this study was to evaluate the impact of seasonal variation on the quantity of euphadienol content (one of the major active constituents) in latex sample of Euphorbia antiquorum L. (EA) and Euphorbia tirucalli L. (ET). Materials and Methods: The latex was collected individually during six different seasons in a calendar year from two species i.e. EA and ET. Then, after suitable sample preparation, it was subjected to high‑performance thin‑layer chromatography study using euphadienol as a standard biomarker and scanned at 210 nm. Results:The euphadienol content was found to be present in all the six seasons across the calendar year. The content was found maximum in Hemanta rutuand Sharada rutu samples in case of EA and ET, respectively. Conclusion: The study proves that changes of seasons do have a certain effect on the phytoconstituents in the plants.

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      Roy, S., Acharya, R., Shukla, V., & Ranade, A. (2020). Effect of Seasonal Variation on Euphadienol Content in Euphorbia antiquorum L. and Euphorbia tirucalli L. using High‑Performance Thin‑Layer Chromatography Method. Pharmacognosy Research, 12(3), 236–242. https://doi.org/10.4103/pr.pr_1_20