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    Gas Chromatography‑High‑Resolution Mass Spectrometry Elucidation and in vitro Cell Line Studies (Sulforhodamine B) on Niosomal Gel of Benincasa hispida

    Gaurav Mahesh Doshi1★, Akanksha Dilip Badgujar2, Ishan Sudhir Mathurvaishya3★ Corresponding author

    1. 1Department of Pharmacology, SVKM'S Dr. Bhanuben Nanavati College of Pharmacy, Mithibai Campus, Mumbai, Maharashtra, INDIA.
    2. 2Department of Quality Assurance, Vivekanand Education, Society's College of Pharmacy, Mumbai, Maharashtra, INDIA.
    3. 3Department of Pharmaceutics, Dr. L.H. Hiranandhani College of Pharmacy, Thane, Maharashtra, INDIA.

    CORRESPONDENCE

    Gaurav Mahesh Doshi

    Department of Pharmacology, SVKM'S Dr. Bhanuben Nanavati College of Pharmacy, Mithibai Campus, Mumbai, Maharashtra, INDIA.

    gaurav.pharmacology@gmail.com

    Received: 26-04-2020; Revised: 26-05-2020; Accepted: 18-08-2020.

    Volume 12, Issue 4 · pp. 342–351 · PUBLISHED · DOI: 10.4103/pr.pr_37_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: The Cucurbitaceae family has been known for its presence of phytoconstituents, namely, lupeol, β-sitosterol, terpenoids, phenols, and proteins with therapeutic potential. Objectives: The research paper tries to focus on major therapeutic field of people demographic area of type of cancer. Soxhlet extraction of Benincasa hispida (BH) was carried out and crude extract was converted to powder. Niosomal gel was formulated after the optimization of drug concentration from the extract of Niosomal solution. Solution with maximum entrapment was selected for the preparation of gel. Gel was prepared by using different Carbopol grades and with comparison of different parameters. Gel was studied for gas chromatography-high-resolution mass spectrometry (GC-HRMS) evaluation for check toxicity. Biologic activity was analyzed by SRB (Sulforhodamine B) assay within vitrocell lines (human oral cancer cell line KB and Mus Musculus skin melanoma B16-F10).Results: Solution which was clear and white was selected for gel development. Carbopol 934 (1') as gelling agent showed good formulation properties. It was non-gritty, easily spreadable, and washable. The gel with maximum entrapment efficiency was evaluated further. GC-HRMS revealed 1.2-ethyl 2-Hexen-1-ol; 1,8-(3-Octyl-2-oxiranyl)-1-octanol; Dodecanoic acid, 2-penten-1-yl ester; Cholestane, 4, 5-epoxy-,(4α,5α); Cyclopentaneundecanoic acid; 17-octadecynoic acid; Octadecanoic acid, methyl ester; Methyl oleate; Tridecanoic acid, methyl ester; and Silane, dimethyl (2,3,6, tricholrophenoxy) heptadicycloxy. The selected cell lines did not show satisfactory activity at different concentrations in SRB assay. Conclusion:This research paper tried to witness the probable potential of BH for cancer with analytical base. Future studies may be undertaken at various stages and levels to further explore the potential of these families of plants.

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      Doshi, G. M., Badgujar, A. D., & Mathurvaishya, I. S. (). Gas Chromatography‑High‑Resolution Mass Spectrometry Elucidation and in vitro Cell Line Studies (Sulforhodamine B) on Niosomal Gel of Benincasa hispida. Pharmacognosy Research, 12(4), 342–351. https://doi.org/10.4103/pr.pr_37_20