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    Pharmacognosy ResearchNOW IN
    Article

    Preparation of Diterpenes‑Enriched Extract from Croton stellatopilosus Ohba Leaves using Enzyme‑ and Ultrasonic‑Assisted Extraction

    Wai Mi Aung1, Supattra Limsuwanchote2, Juraithip Wungsintaweekul1★★ Corresponding author

    1. 1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, THAILAND.
    2. 2Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, THAILAND.

    CORRESPONDENCE

    Juraithip Wungsintaweekul

    Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, THAILAND.

    juraithip.w@psu.ac.th

    Received: 07-10-2020; Accepted: 16-03-2021.

    Volume 13, Issue 1 · pp. 22–27 · PUBLISHED 27 April 2021 · DOI: 10.4103/pr.pr_90_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Croton stellatopilosus Ohba accumulates several diterpenes. Among them, plaunotol, a cytoprotective agent, has been prescribed to treat peptic ulcers. Objectives: We aimed to prepare the diterpene‑enriched extract (DEE) using enzyme‑ and ultrasonic‑assisted extractions. Materials and Methods:The type and amount of pectinase and cellulase and the condition of ultrasonic treatment were optimized. A combination of enzyme and ultrasonic treatments was performed. The high‑performance liquid chromatography (HPLC) method was validated and used for the determination of plaunotol and plaunolide contents in the DEE. Results:A combination of cellulase (10 units) and ultrasonic (at 80% amplitude) treatments enhanced the yield of plaunotol significantly. Application of the crude extract on diaion HP‑20 resin afforded the DEE. With the validated HPLC method, the DEE contained 8.81% ± 0.02% w/w of plaunotol and 8.40% ± 0.92% w/w of plaunolide. Conclusion:We established an efficient method to prepare DEE based on green extraction. This method showed about eightfold enhancement purification factor compared to the original crude extract and about 90% yield recovery.

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      Aung, W. M., Limsuwanchote, S., & Wungsintaweekul, J. (2021). Preparation of Diterpenes‑Enriched Extract from Croton stellatopilosus Ohba Leaves using Enzyme‑ and Ultrasonic‑Assisted Extraction. Pharmacognosy Research, 13(1), 22–27. https://doi.org/10.4103/pr.pr_90_20