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    Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model

    Lidiane Schultz Branquinho1, Maria Helena Verdan2★, Saulo Euclides Silva-Filho3, Rodrigo Juliano Oliveira4, Claudia Andrea Lima Cardoso5, Arielle Cristina Arena6, Candida Aparecida Leite Kassuya1★ Corresponding author

    1. 1Faculty of Health Sciences, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
    2. 2Faculty of Exact Sciences and Technology, Postgraduate Program in Chemistry, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
    3. 3Faculty of Pharmaceutical Sciences, Federal University of Mato Grosso do Sul, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
    4. 4Faculty of Medicine (FAMED) “Dr. Helio Mandetta”, Federal University of Mato Grosso do Sul,, Campo Grande, MS, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
    5. 5Center of Studies in Natural Resources, State University of Mato Grosso do Sul, Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.
    6. 6Department of Morphology, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.

    CORRESPONDENCE

    Maria Helena Verdan

    Faculty of Exact Sciences and Technology, Postgraduate Program in Chemistry, Federal University of Grande Dourados, Institute of Biosciences of Botucatu, Paulista State University, Botucatu, SP, BRAZIL.

    mhelenaverdan@gmail.com

    Received: 07-08-2020; Revised: 05-10-2020; Accepted: 05-01-2021.

    Volume 13, Issue 1 · pp. 28–33 · PUBLISHED 27 April 2021 · DOI: 10.4103/pr.pr_79_20

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Objectives:The folk use of Doliocarpus dentatus for pain and inflammatory conditions led our group to evaluate the anti‑inflammatory, antinociceptive, and antiarthritic effects of its ethanol extract from the leaves (EEDd) on mouse models. Results: Oral treatments with EEDd (100–300 mg/kg) significantly inhibited the formalin‑induced nociceptive and cold sensitivity, prevented acetic acid‑induced nociceptive behavior, and prevented articular inflammation (including knee edema, leukocyte infiltration, and mechanical hyperalgesia) induced by zymosan. In the peritonitis model, betulinic acid (BA, 0.3–30 mg/kg) and EEDd (300 mg/kg) significantly inhibited zymosan‑induced leukocyte infiltration. In the complete Freund adjuvant (CFA) model, oral treatments with EEDd (100–300 mg/kg) for 21 days significantly inhibited mechanical hyperalgesia, cold response, and edema. In the MTT viability assay, EEDd (3–90 μg/mL) did not induce leukocytes cytotoxicity. cytotoxicity. Most models employed male and female Swiss mice or, for the CFA test, C57BL/6 mice. Conclusion:This study demonstrates that EEDd exhibited antinociceptive, antihyperalgesic, and antiarthritic potential in mice and BA contribute for the EEDd observed activities.

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      Branquinho, L. S., Verdan, M. H., Silva-Filho, S. E., Oliveira, R. J., Cardoso, C. A. L., Arena, A. C., & Kassuya, C. A. L. (2021). Antiarthritic and Antinociceptive Potential of Ethanolic Extract from Leaves of Doliocarpus dentatus (Aubl.) Standl. in Mouse Model. Pharmacognosy Research, 13(1), 28–33. https://doi.org/10.4103/pr.pr_79_20