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    Article

    Anticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves

    Enrico Rogatto Rovetta1, Mara Angelina Galvão Magenta2, Thaís Moura Gascon3, Fernando Luiz Affonso Fonseca3, Robson Miranda da Gama1, José Armando Jr1, Horácio Dorigan Moya1★★ Corresponding author

    1. 1Centro Universitário FMABC, Pharmacy Laboratory, Santo André, São Paulo, BRAZIL.
    2. 2Universidade Santa Cecília, Santos, São Paulo, BRAZIL.
    3. 3Centro Universitário FMABC, Clinical Analysis Laboratory, Santo André, São Paulo, BRAZIL.
    4. 4Centro Universitário FMABC, Analytical Chemistry Laboratory, Santo André, São Paulo, BRAZIL.

    CORRESPONDENCE

    Horácio Dorigan Moya

    Centro Universitário FMABC, Pharmacy Laboratory, Santo André, São Paulo, BRAZIL.

    horacio.moya@fmabc.br

    Received: 03-12-2021; Revised: 23-02-2021; Accepted: 15-05-2021.

    Volume 13, Issue 3 · pp. 121–128 · PUBLISHED 1 June 2021 · DOI: 10.5530/pres.13.3.3

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Ischemic diseases are one of the leading causes of death worldwide; therefore, the search for new compounds with safer and more effective anticoagulant and antiplatelet action becomes relevant. Objectives:To evaluate the impact of Dalbergia ecastaphyllum (L.) Taub. dry leaf extract, a species of the Fabaceae family that is commonly found in Brazil, on blood coagulation. Materials and Methods: A statistical design of experiments was carried out with selection of five factors and two levels, resulting in 32 different ethanolic raw extracts. The extracts were resuspended in saline and tested in a pool of human blood. The activated partially thromboplastin time (APTT) and the prothrombin time (PT) were analysed in a Thrombolyzer®. Results:Treatment using ethanol 70%, a drug/solvent ratio of 5%, pH 9 and dynamic maceration for 6 hrs resulted in the best combination for the extraction of total phenols, while ethanol 70%, a drug/solvent ratio of 5% and dynamic maceration for 24 hrs showed the best flavonoid extraction, with the pH being inconclusive. On average, all extracts were capable of increasing both APTT and PT, especially those in lower dilutions (1:5); however, the overall efficacy of the extracts is inconclusive.Conclusion: The species under study showed important results in the blood clotting process in vitro, increasing the mean time of APTT and PT, which suggests that Dalbergia ecastaphyllum (L.) Taub. has some potential as a possible drug in the anticoagulant class.

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      Rovetta, E. R., Magenta, M. A. G., Gascon, T. M., Fonseca, F. L. A., Gama, R. M. D., Jr, J. A., & Moya, H. D. (2021). Anticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves. Pharmacognosy Research, 13(3), 121–128. https://doi.org/10.5530/pres.13.3.3