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    Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos

    Thiago Felix da Silva1★, Maria Isabel de Assis Lima Castro2, Marta Gerusa Soares de Lucena3, Camilla Vila Nova Soares Silva4, Laisla Rangel Peixoto5, Eliane Alves Bandeira de Carvalho6, Silvania Tavares Paz7, Beathryz Lethycya Almeida Guimarães3, José Maria Barbosa Filho8, Eliete Cavalcanti da Silva1★ Corresponding author

    1. 1Postgraduate Program in Morphotechnology - Federal University of Pernambuco, Recife, PE, BRAZIL.
    2. 2Master of Sciences; Postgraduate Program in Biological Sciences - Federal University of Pernambuco, Recife, PE, BRAZIL.
    3. 3Department of Histology and Embryology - Federal University of Pernambuco, Recife, PE, BRAZIL.
    4. 4Multiprofessional Residency Program in Health Surveillance - Recife Health Department, Recife, PE, BRAZIL.
    5. 5Master of Sciences; Institute of Drugs and Medicines Research - Federal University of Paraíba, João Pessoa, PB, BRAZIL.
    6. 6Master of Sciences; Postgraduate Program in Biological Sciences - Federal University of Pernambuco, Recife, PE, BRAZIL.
    7. 7Master of Sciences; Department of Pathology - Federal University of Pernambuco, Recife, PE, BRAZIL.
    8. 8Institute of Drugs and Medicines Research - Federal University of Paraíba, João Pessoa, PB, BRAZIL.

    CORRESPONDENCE

    Thiago Felix da Silva

    Postgraduate Program in Morphotechnology - Federal University of Pernambuco, Recife, PE, BRAZIL.

    thiagofelix.felix@hotmail.com

    Received: 30-03-2021; Revised: 15-05-2021; Accepted: 04-06-2021.

    Volume 13, Issue 3 · pp. 135–139 · PUBLISHED 10 June 2025 · DOI: 10.5530/pres.13.3.5

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Introduction: Yangambin, a lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal. Objectives: The aim of this study was to evaluate the embryotoxicity of yangambin on the neurodevelopment of Gallus gallus domesticus embryos. Materials and Methods: 120 fertilized eggs were divided in three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 μg/ml yangambin) and G3 (65 μg/ml yangambin) and each egg was inoculated with 100 μL of the respective solutions. The fertilized eggs were incubated at a temperature of 37.5°C, with a relative humidity of 65% to 75%, for 48 hr and then their embryos were histologically processed. Results: In staging, carried out according to Hamburger and Hamilton (1951), variations of stages were identified. In all groups, the morphological analysis revealed the closure of the anterior neuropore and absence of malformations in the optic vesicles and in the secondary encephalic vesicles. In the caudal region, a standard development of the neural tube was observed, with well-segmented somites and regression of the primitive line. The cross sections showed that the internal structure of the somite’s, composed of dermatome, myotome and sclerotome, was preserved. The statistical analysis did not show significant differences between the groups regarding the morphometry of the cephalic and caudal regions of the neural tube. Conclusion: Yangambin did not show embryotoxic effects on the neurodevelopment of Gallus gallus domesticus embryos, under the tested conditions.

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      Silva, T. F. D., Castro, M. I. D. A. L., Lucena, M. G. S. D., Silva, C. V. N. S., Peixoto, L. R., Carvalho, E. A. B. D., Paz, S. T., Guimarães, B. L. A., Filho, J. M. B., & Silva, E. C. D. (2025). Embryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos. Pharmacognosy Research, 13(3), 135–139. https://doi.org/10.5530/pres.13.3.5