Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

ABSTRACT

Background:According to a literature review Tinospora cordifoliapossess alkaloids, tannins, flavonoids, glycosides, and steroids with anti-cancer potential. However, there is a lack of scientific evidence on the green synthesis of selenium nanoparticles from the stem of T. cordifolia and its biological activities. Objectives: This study is intended to synthesize Selenium Nanoparticles using T. cordifoliastem extract, characterise them, and evaluate them in-vitro for antioxidant and anticancer activity. Materials and Methods:TheT. cordifoliaSelenium Nanoparticles (TC-SeNPs) were synthesised by reducing selenious acid withT. cordifolia stem extract. TC-SeNPs were characterized by UV–Vis, FTIR, XRD, FE-SEM, EDAX and ICP-AES, further evaluated for in-vitro antioxidant activity by DPPH free radical scavenging test and reducing power capacity. The MTT assay of TC-SeNP was carried out in-vitro in the human breast cancer cells line (MCF-7) to determine the anticancer activity.Results:The UV-spectroscopy of TC-SeNPs showed maximum absorbance at 285 nm, which is the characteristic feature of SeNP. According to DLS, SEM, and TEM images, the size of TC-SeNPs was between 100-200 nm and was stable with a Zeta potential of -23.9mV. XRD analysis confirmed the crystallinity of TC-SeNPs. Selenium in TC-SeNP was verified in EDAX analysis (63.36% weight) and ICP-AES 68.08 ±12.2 μg/ml selenium content. The IC50 value 28.62 ± 0.63 μg/mL and EC50 (62.50 ± 1.21 μg/mL) values indicated that the TC-SeNPs possess significant antioxidant capacity. MTT assay showed TC-SeNPs were cytotoxic to MCF-7 cell with IC50 values (31.29 ± 0.22μg/mL), suggesting that TC-SeNPs display moderate cytotoxicity that could dose-dependently inhibit cell proliferation. Conclusion: Thus, experimental evidence provides insight into the successful synthesis of the selenium nanoparticle from the stem of T. c

KEYWORDS

• Anticancer
• Antioxidant
• Green synthesis
• Phytonanoparticles
• Selenium nanoparticles
• Tinospora cordifolia

Showing references and in-text citations exactly as published.

Cite this article

• DOI10.5530/pres.14.3.42Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 14, ISSUE 3Comparative Analysis of Antidermatophytic Potential of Cymbopogon citratus Essential Oil Stored in Different Temperature Condition for Two Years ArticlesNeetu Jain, Meenakshi Sharma · pp. 241–245Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 3In silico Investigation on Phytoconstituents in Pamburus missionis S. for Antioxidant ActivityRaveesha Peeriga, Keerthi Priyanka Adarapu, Akhila Kurama, Naseemunnisa Mohammed, Lakshmana Rao Atmakuri, Deepak Kumar · pp. 246–250Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 3Clinical Assessment of Antimicrobial Effects of Brazilian Green Propolis on Chronic WoundsPascalle Sousa Rocha, Fernando Luiz Affonso Fonseca, David Feder, Luiz Elidio Gregório, Joserlan Nonato Moreira, Luis Rafael Leite Sampaio, Glaucia Luciano da Veiga, Fulvio Alexandre Scorza, Beatriz da Costa Aguiar Alves, Thais Gascón, Carla Alessandra Scorza, Fabio Ferreira Perazzo · pp. 251–255Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 3KRAS G12C as a Target of Naringenin for Inducing Cell Death in NCI-H23 CellsAbani Kumar Patar, Lakhon Kma, Jitul Barman, Shekhar Ghosh, Taranga Jyoti Baruah · pp. 256–262Original ArticleSame type: Article