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    Pharmacognostic Evaluation and Development of Quality Control Parameters for Root of Abelmoschus manihot (L.) Medik

    Deshraj Shyamkant Chumbhale1, Mahendra Shivshankar Khyade2★★ Corresponding author

    1. 1Department of Pharmacognosy, Amrutvahini College of Pharmacy, Sangamner, Ahmednagar, Maharashtra, INDIA.
    2. 2Department of Botany, Sangamner Nagarpalika Arts, D.J. Malpani Commerce and B.N. Sarda Science College (Autonomous), Sangamner, Maharashtra, INDIA.

    CORRESPONDENCE

    Mahendra Shivshankar Khyade

    Department of Botany, Sangamner Nagarpalika Arts, D.J. Malpani Commerce and B.N. Sarda Science College (Autonomous), Sangamner, Maharashtra, INDIA.

    maheshkhyade@gmail.com

    Received: 13-09-2022; Revised: 23-10-2022; Accepted: 09-11-2022.

    Volume 15, Issue 1 · pp. 101–111 · PUBLISHED · DOI: 10.5530/097484900263

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:In traditional medicine, Abelmoschus manihot(L.) Medik, which belongs to the Malvaceae family, has been used to treat boils, sores, sprains, inflammations, tuberculosis, and leucoderma. Objectives: The objective of this study was to establish identification and quality control standards. Materials and Methods: For pharmacognostic evaluation, macroscopy, micromorphology and physicochemical constants have been used. Microchemical colour reaction tests and HPTLC studies were performed for qualitative phytochemistry. Results: Externally the root is yellowish-brown to yellowish-white and has a distinct odour, bitter taste, wavy shape, and smooth texture. The periderm was composed of 4-5 thin-walled cells. The secondary phloem has dilated rays and broad pyramid-shaped mucilage-packed rays. The fibres have thin walls and are narrow, and the secondary xylem is a compact circular cylinder with eight to eight radial lines of vessel chains. Prismatic calcium oxalate crystals, lignified long, narrow, thick-walled fibres with parenchyma cells, lignified cork cells, and cylindrical vessel elements were discovered in powdered samples. Physicochemically, the ash value was found above 8% which shows more than 75% solubility in acid whereas water and methanol extractive values were found to be just above and below 8% respectively. In UV-fluorescence study, very distinctive colour changes of the powdered root have been recorded. The phytochemical analysis reveals the presence of glycosides, saponins, flavonoids, tannins, and steroids. GC-MS and HPTLC are also used to characterize lipid and phenolic molecules. Conclusion: These findings will help in the future identification and quality control ofA. manihot as reference standards.

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      Chumbhale, D. S., & Khyade, M. S. (). Pharmacognostic Evaluation and Development of Quality Control Parameters for Root of Abelmoschus manihot (L.) Medik. Pharmacognosy Research, 15(1), 101–111. https://doi.org/10.5530/097484900263