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    Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L

    G. M. Gururaja1,2★, Deepak Mundkinajeddu2, Shekhar M. Dethe2, Gopala K. Sangli2, K. Abhilash2, Amit Agarwal2★ Corresponding author

    1. 1Research Scholar, Manipal University, Manipal
    2. 2R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India

    CORRESPONDENCE

    G. M. Gururaja

    *Address for correspondence: Mr. G. M. Gururaja, Natural Remedies Pvt, Ltd., Bengaluru, Karnataka, India.

    gururajphyto@gmail.com

    Revised: 10-12-2014.

    Volume 7, Issue 4 · pp. 355–362 · PUBLISHED October-December 2015 · DOI: 10.4103/0974-8490.159578

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far. Objective To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract. Materials and Methods The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds. Results and Discussion A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86µg/ml.Conclusion: This study demonstrates that M.indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

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      Gururaja, G. M., Mundkinajeddu, D., Dethe, S. M., Sangli, G. K., Abhilash, K., & Agarwal, A. (2015). Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L. Pharmacognosy Research, 7(4), 355–362. https://doi.org/10.4103/0974-8490.159578