Chemical Characterization and Cytoprotective Effect of the Hydroethanol Extract from Annona coriacea Mart. (Araticum)
José G. A. S. Júnior1, Henrique D. M. Coutinho2★, Ticiana C. C. Boris1, Janyketchuly S. Cristo1, Nara L. F. Pereira1, Fernando G. Figueiredo3, Francisco A. B. Cunha2, Pedro E. A. Aquino4, Polyana A. C. Nascimento3, Francisco J. C. Mesquita1, Paulo H. F. Moreira1, Sáskia T. B. Coutinho3, Ivon T. Souza1, Gabriela C. Teixeira5, Najla M. N. Ferreira1, Eleonora O. Farina1, Cícero M. G. Torres6, Vanderlan N. Holanda6, Vandbergue S. Pereira4, Maria I. F. Guedes1★ Corresponding author
- 1Department of Biotechnology, Ceará State University, Fortaleza (CE)
- 2Department of Biological Chemistry, Regional University of Cariri, Crato (CE)
- 3Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE)
- 4Department of Medical Microbiology, Federal University of Ceará, Fortaleza (CE)
- 5Department of Medicine, Medical School of Juazeiro do Norte - Estacio, Juazeiro do Norte (CE)
- 6Department of Health, University of Fortaleza – UNIFOR, Fortaleza (CE) Brazil
CORRESPONDENCE
Henrique D. M. Coutinho
Departamento de Química Biológica, Universidade Regional Do Cariri, Av. Cel. Antônio Luiz, 1161, CEP: 63105-000, Crato, CE, Brazil.
Volume 8, Issue 4 · pp. 253–257 · PUBLISHED Oct-Dec 2016 · DOI: 10.4103/0974-8490.188882
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ABSTRACT
Introduction Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride. Materials and Methods The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8). Results and Discussion The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 mg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P< 0.0001) and E. coli(significance P< 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification. Conclusions The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.
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Júnior, J. G. A. S., Coutinho, H. D. M., Boris, T. C. C., Cristo, J. S., Pereira, N. L. F., Figueiredo, F. G., Cunha, F. A. B., Aquino, P. E. A., Nascimento, P. A. C., Mesquita, F. J. C., Moreira, P. H. F., Coutinho, S. T. B., Souza, I. T., Teixeira, G. C., Ferreira, N. M. N., Farina, E. O., Torres, C. M. G., Holanda, V. N., Pereira, V. S., & Guedes, M. I. F. (2016). Chemical Characterization and Cytoprotective Effect of the Hydroethanol Extract from Annona coriacea Mart. (Araticum). Pharmacognosy Research, 8(4), 253–257. https://doi.org/10.4103/0974-8490.188882