Anti-Advanced Glycation End-product and Free Radical Scavenging Activity of Plants from the Yucatecan Flora

ABSTRACT

Background Formation and accumulation of advanced glycation end- products (AGE) is recognized as a major pathogenic process in diabetic complications, atherosclerosis and cardiovascular diseases. In addition, reactive oxygen species and free radicals have also been reported to participate in AGE formation and in cell damage. Natural products with antioxidant and antiAGE activity have great therapeutic potential in the treatment of diabetes, hypertension and related complications. Objective to test ethanolic extracts and aqueous-traditional preparations of plants used to treat diabetes, hypertension and obesity in Yucatecan traditional medicine for their anti-AGE and free radical scavenging activities. Materials and Methods ethanolic extracts of leaves, stems and roots of nine medicinal plants, together with their traditional preparations, were prepared and tested for their anti-AGE and antioxidant activities using the inhibition of advanced glycation end products and DPPH radical scavenging assays, respectively. Results the root extract of C. fistula (IC50= 0.1 mg/mL) and the leaf extract of P. auritum (IC50= 0.35 mg/mL) presented significant activity against vesperlysine and pentosidine-like AGE. Although none of the aqueous traditional preparations showed significant activity in the anti- AGE assay, both the traditional preparations and the ethanolic extracts of E. tinifolia, M. zapota, O. campechianum and P. auritum showed significant activity in the DPPH reduction assay. Conclusions the results suggest that the metabolites responsible for the detected radical-scavenging activity are different to those involved in inhibiting AGE formation; however, the extracts with antioxidant activity may contain other metabolites which are able to prevent AGE formation through a different mechanism.

KEYWORDS

• Antioxidant activity
• diabetes
• glycation-end products
• traditional medicine

Showing references and in-text citations exactly as published.

• DOI10.4103/0974-8490.188883Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Res..

• Article · VOLUME 8, ISSUE 4In vivo Study on Depressant Effects and Muscle Coordination Activity of Galphimia glauca Stem Methanol ExtractBaba Shankar Rao Garige, Srisailam Keshetti, Uma Maheshwara Rao Vattikuti · pp. 219–225ORIGINAL ARTICLESame type: Article
• Article · VOLUME 8, ISSUE 4Evaluation of Antiulcer and Antioxidant Activity of Barleria gibsoni Dalz. LeavesFiroj A. Tamboli, Harinath N. More · pp. 226–230ORIGINAL ARTICLESame type: Article
• Article · VOLUME 8, ISSUE 4Probing Regenerative Potential of Moringa oleifera Aqueous Extracts Using In vitro Cellular AssaysEvangeline E. Fernandes, Anubha V. Pulwale, Gauri A. Patil, Alpana S. Moghe · pp. 231–237ORIGINAL ARTICLESame type: Article
• Article · VOLUME 8, ISSUE 4High-performance Thin-layer Chromatography Method Development, Validation, and Simultaneous Quantification of Four Compounds Identified in Standardized Extracts of Orthosiphon stamineusSuzana Hashim, Hooi Kheng Beh, Mohamad Shahrul Ridzuan Hamil, Zhari Ismail, Amin Malik Shah Abdul Majid · pp. 238–243ORIGINAL ARTICLESame type: Article