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    Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams

    Komal Kumar Javarappa1★, Attemode Girijanna Devi Prasad2, AJ Mahadesh Prasad3, Chetana Mane4★ Corresponding author

    1. 1Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden
    2. 2Department of Environmental Science
    3. 3Department of Biochemistry, PBM Mahajana PG Centre, Mysore
    4. 4Department of Biochemistry, University of Mysore, Karnataka, India

    CORRESPONDENCE

    Komal Kumar Javarappa

    Department of Clinical and Experimental Medicine, Experimental Haematology Unit, University of Linkoping, Linkoping, Sweden.

    kompaddy@yahoo.com

    Volume 8, Issue 4 · pp. 287–291 · PUBLISHED Oct-Dec 2016 · DOI: 10.4103/0974-8490.188871

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background Kingiodendron pinnatum Rox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation of K. pinnatum has not been established so far though it is being traditionally used by tribes of the region. Objective P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract of Kingiodendron pinnatum Rox. Hams. Materials and Methods Chromatographic separation was carried out by thin layer chromatography and column chromatography. Bio-autography of the column fractioned extract and TLC chromatogram were evaluated in vitro for antibacterial activity. The PTLC, HP TLC were used for crude extract and HPLC, LCMS, FTIR 1HNMR and 13CNMR were employed for the isolated compound in the ethyl acetate extract of K. pinnatum. Results Evaluation of solvent system for chromatographic separation revealed that ethyl acetate: petroleum ether in the ratio of 7:2.5 ml was the most appropriate one for the separation of diterpene compounds. The antibacterial bio-autography screening of TLC separated compound showed positive activity with Staphylococcus aureus and negative activity with Escherichia coli. Spectroscopic analysis of the isolated compound from the ethyl acetate extract of K. pinnatum revealed the presence of diterpene compound. Conclusion It is evident from the present study that the ethyl acetate extract of K. pinnatum is rich in diterpene compounds and having potential antibacterial activity.

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      Javarappa, K. K., Prasad, A. G. D., Prasad, A. M., & Mane, C. (2016). Bioactivity of Diterpens from the Ethyl Acetate Extract of Kingiodendron pinnatum Rox. Hams. Pharmacognosy Research, 8(4), 287–291. https://doi.org/10.4103/0974-8490.188871