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    Antihyperglycemic and Insulin Secretagogue Activities of Abrus precatorius Leaf Extract

    Balekari Umamahesh1, Ciddi Veeresham1★★ Corresponding author

    1. 1Department of Pharmacognosy, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India

    CORRESPONDENCE

    Ciddi Veeresham

    University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009, Telangana, India.

    ciddiveeresham@yahoo.co.in

    Volume 8, Issue 4 · pp. 303–308 · PUBLISHED Oct-Dec 2016 · DOI: 10.4103/0974-8490.188881

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Aim Abrus precatorius leaves methanolic extract (APME) was evaluated for in vivo antihyperglycemic activity and in vitro insulinotropic effect. Materials and Methods In vivo antihyperglycemic and insulin secretagogue activities were assessed in streptozotocin-induced diabetic rats by oral administration of APME (200 mg/kg body weight [bw]) for 28 days. In vitro insulin secretion mechanisms were studied using mouse insulinoma beta cells (MIN6-b). In vivo body weight and blood glucose and in vivo and in vitro insulin levels were estimated. Results In diabetic rats, APME treatment significantly restored body weight (26.39%), blood glucose (32.39%), and insulin levels (73.95%) in comparison to diabetic control rats. In MIN6-b cells, APME potentiated insulin secretion in a dependent manner of glucose (3–16.7 mM) and extract (5–500 μg/mL) concentration. Insulin secretagogue effect was demonstrated in the presence of 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium, and K+ depolarized media. Insulin release was reduced in the presence of nifedipine, ethylene glycol tetra acetic acid (calcium blocking agents), and diazoxide (potassium channel opener). Conclusion The study suggests that APME antihyperglycemic activity might involve the insulin secretagogue effect by pancreatic beta cells physiological pathways via K+-ATP channel dependent and independently, along with an effect on Ca2+ channels.

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      Umamahesh, B., & Veeresham, C. (2016). Antihyperglycemic and Insulin Secretagogue Activities of Abrus precatorius Leaf Extract. Pharmacognosy Research, 8(4), 303–308. https://doi.org/10.4103/0974-8490.188881