Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Pharmacognostic Specification, Chlorogenic Acid Content, and In vitro Antioxidant Activities of Lonicera japonica Flowering Bud

    Chayanon Chaowuttikul1, Chanida Palanuvej1★, Nijsiri Ruangrungsi2★ Corresponding author

    1. 1Public Health Sciences Program,College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, THAILAND.
    2. 2Public Health Sciences Program,College of Public Health Sciences, Chulalongkorn University, Bangkok 10330; Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, THAILAND.

    CORRESPONDENCE

    Chanida Palanuvej

    Public Health Sciences Program,College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, THAILAND.

    chanida.p@chula.ac.th

    Volume 9, Issue 2 · pp. 128–132 · PUBLISHED 1 April 2017 · DOI: 10.4103/pr.pr_117_16

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Lonicera japonica Thunb. or Japanese Honeysuckle has been widely used in traditional medicine for antipyretic. Objective: To establish the pharmacognostic specification ofL. japonicaflowering bud in Thailand and to determine its chlorogenic acid content and in vitro antioxidant activities.Materials and Methods:Dried L. japonica flowering bud from 15 various herbal drugstores throughout Thailand were investigated for pharmacognostic specification. Their chlorogenic acid contents were quantitatively analyzed by thin layer chromatography (TLC) densitometry with winCATS software. The mobile phase for TLC development consisted of ethyl acetate: formic acid: acetic acid: water (10:1.1:1.1:2.6). Antioxidant activities were investigated by 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) radical scavenging, ferric ion reducing antioxidant power assay, nitric oxide scavenging assay, and β‑carotene bleaching assays. Results: QualifiedL. japonicaflowering bud in Thailand was presented that the contents of loss on drying, total ash, acid‑insoluble ash, and water should not be >10.11%, 6.59%, 1.14%, and 10.82% by weight, respectively. The ethanol and water soluble extractive values should not be <16.46% and 28.88% by weight, respectively. Chlorogenic acid content inL. japonica flowering bud was found to be 2.24 ± 0.50 g/100 g of crude drug. L. japonicaflowering bud showed DPPH and nitric oxide scavenging activities as well as reducing power property.Conclusion:This pharmacognostic specification with special reference to the chlorogenic acid content can be used for quality control of L. japonicaflowering bud in Thailand. The potential antioxidant of this crude drug was demonstrated in vitro.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Chaowuttikul, C., Palanuvej, C., & Ruangrungsi, N. (2017). Pharmacognostic Specification, Chlorogenic Acid Content, and In vitro Antioxidant Activities of Lonicera japonica Flowering Bud. Pharmacognosy Research, 9(2), 128–132. https://doi.org/10.4103/pr.pr_117_16