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    Free Radicals and AntioxidantsNOW IN
    Article

    Orientin Protects BV-2 Microglial Cells against Hypoxia Reoxygenation Injury through Inhibiting Oxidative Stress

    Siqun Jing1★, Yixian Tu2, Hongyue Zhai2, Junyan Zhang1, Genmei Liu1, Jinsheng Cheng1, Suhua Qi3, Yun-Guo Liu4, Liang-Jun Yan5★ Corresponding author

    1. 1Department of Food Science and Engineering, Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region; College of Food, Shaoguan University, Daxue Road 288, Shaoguan, Guangdong, PR CHINA.
    2. 2Department of Food Science and Engineering, College of Life Sciences and Technology, Xinjiang University, Shengli Road 14, Urumqi, Xinjiang, PR CHINA.
    3. 3Research Center for Biochemistry and Molecular Biology and Jiangsu Key Laboratory of Brain Disease Bioinformation, School of Medical College, Xuzhou Medical University, Xuzhou, China, PR CHINA.
    4. 4College of Life Sciences, Linyi University, Linyi, CHINA.
    5. 5Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Boulevard, Fort Worth, TX, USA.

    CORRESPONDENCE

    Siqun Jing

    Department of Food Science and Engineering, Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region; College of Food, Shaoguan University, Daxue Road 288, Shaoguan, Guangdong, PR CHINA.

    jingsiqun@163.com

    Received: 21-08-2023; Revised: 03-09-2023; Accepted: 30-09-2023.

    Volume 13, Issue 2 · pp. 60–66 · PUBLISHED · DOI: 10.5530/fra.2023.2.11

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Background: Oxidative stress is one of the pivotal pathogenesis of ischemic stroke. Orientin, a compound extracted from plants, has been previously reported to have antioxidant activity that was exploited in various diseases. The objective of this study was to focus on the potential therapeutic effects of orientin on cobalt chloride (CoCl2)-induced Hypoxia Reoxygenation (HR) injury in BV-2 microglial cells and to explore the possible mechanisms. Materials and Methods: The optimum protective condition of orientin on BV-2 microglial cells after HR injury was evaluated by the viability of cells measured by Cell Counting Kit-8 (CCK-8). Afterwards, functions of Superoxide Dismutase (SOD) and Catalase (CAT) as well as the content of Glutathione Peroxidase (GSH-PX) were measured by corresponding kits. Activation of Nrf2 pathway was assayed by western blotting. Results:Our study demonstrates that the viability of HR injured BV-2 microglial cells was improved significantly after incubation with orientin (40~100 μg/mL) for 8 hr. Furthermore, orientin was found to increase the nuclear translocation of Nrf2 protein in BV-2 microglial cells and was shown to promote antioxidant enzyme activities and enhance the expression of HO-1 and NQO1. Conclusion: Taken together, Results of our study indicate that orientin protect BV-2 microglial cells against HR injury induced by CoCl2, and this protection is associated with the inhibition of oxidative stress through the activation of the Nrf2 signaling pathway.

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      Jing, S., Tu, Y., Zhai, H., Zhang, J., Liu, G., Cheng, J., Qi, S., Liu, Y., & Yan, L. (). Orientin Protects BV-2 Microglial Cells against Hypoxia Reoxygenation Injury through Inhibiting Oxidative Stress. Free Radicals and Antioxidants, 13(2), 60–66. https://doi.org/10.5530/fra.2023.2.11