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    Anti-Parkinson Potential of Indian Ocimum species in Relation to Active Components as Revealed Using Metabolites Profiling, in vitro and in silico Enzyme Inhibition Studies

    Sreerupa Sarkar1, Jhelam Chatterjee1, Aditi Gangopadhyay2, Mohammed Sheashea3, Mohamed A. Farag4, Achintya Saha2, Susmita Das1, Bratati De1★★ Corresponding author

    1. 1Department of Botany, Phytochemistry and Pharmacognosy Research Laboratory, University of Calcutta, Kolkata, West Bengal, INDIA.
    2. 2Department of Chemical Technology, Drug Design and Cheminformatics Laboratory, University of Calcutta, Kolkata, West Bengal, INDIA.
    3. 3Department of Aromatic and Medicinal Plants, Desert Research Center, Cairo, EGYPT.
    4. 4Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, EGYPT.

    CORRESPONDENCE

    Bratati De

    Department of Botany, Phytochemistry and Pharmacognosy Research Laboratory, University of Calcutta, Kolkata, West Bengal, INDIA.

    bratatide@hotmail.com

    Received: 17-11-2023; Revised: 02-12-2023; Accepted: 22-01-2024.

    Volume 13, Issue 2 · pp. 74–85 · PUBLISHED · DOI: 10.5530/fra.2023.2.13

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Background:Parkinson’s Disease (PD) is a debilitating neurodegenerative disease suffered by elderly population worldwide. There are different treatment options for its symptomatic relief including the use of inhibitors of monoamine oxidase-B, acetylcholinesterase enzymes and L-DOPA though with certain side effects. Materials and Methods:To identify new resources of natural products with potential effects to treat PD, several Indian Ocimum species, e.g., O. tenuiflorum (green), O. tenuiflorum (purple), O. basilicum, O. americanum, O. kilimandscharicum, and O. gratissimum were screened. Activities of the extracts were studied against monoamine Oxidase-B (MAO-B), and acetylcholinesterase (AChE). Identification of active components present in these extracts was determined using GC-MS and LC-MS metabolites profiling. Further, activity of identified phenolics was measured by docking score against MAO-B and AChE. Results: All extracts and several phenolics showed potential inhibition of these two PD related enzymes based on in vitro and in silico, respectively. Ellagic acid-di-methyl ether-O-glucoside, rosmarinic acid, and luteolin-5-glucuronide were identified as top scoring hits against both AChE and MAO-B from molecular docking studies. L-DOPA was also detected in all the species of Ocimum. Conclusion: IC50 values against both enzymes and L-DOPA levels detected in O. tenuiflorum (green), O. basilicum, and O. americanum pose them as potential nutraceuticals for PD treatment.

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      Sarkar, S., Chatterjee, J., Gangopadhyay, A., Sheashea, M., Farag, M. A., Saha, A., Das, S., & De, B. (). Anti-Parkinson Potential of Indian Ocimum species in Relation to Active Components as Revealed Using Metabolites Profiling, in vitro and in silico Enzyme Inhibition Studies. Free Radicals and Antioxidants, 13(2), 74–85. https://doi.org/10.5530/fra.2023.2.13