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    Free Radicals and AntioxidantsNOW IN
    Article

    Modulation of redox homeostasis by Lamiaceae herbs in seminal vesicles of Lumbricus terrestris

    Dhiraj Anil Vattem1★, Robert Corey DeLeon1★ Corresponding author

    1. 1Nutrition Biomedicine and Biotechnology Laboratory, School of Family and Consumer Sciences, Texas State University, San Marcos, TX 78666, USA.

    CORRESPONDENCE

    Dhiraj Anil Vattem

    Nutrition Biomedicine and Biotechnology Laboratory, School of Family and Consumer Sciences, Texas State University, San Marcos, TX 78666, USA.

    dv11@txstate.edu

    Received: 27-03-2013; Revised: 23-05-2013; Accepted: 20-08-2013.

    Volume 3, Issue 2 · pp. 93–99 · PUBLISHED · DOI: 10.1016/j.fra.2013.05.003

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Background/Aims:Health benefits of Lamiaceae herbs are attributed to the presence of antioxidant phytochemicals. However, mechanism of in vivo functionality is not very well understood. We deter- mined the in vivo effect of dietary Lamiaceae herbs on modulating redox-nitric oxide (NO) homeostasis and sperm quality in oxidation prone environment of seminal vesicles in Lumbricus terrestris. Methods:Animals fed ad libitum on Lumbricus growth medium supplemented with 0% (control), 0.1% or 0.5% (w/v) of different herbs. The seminal vesicles of the animal were dissected out on day 2 and day 6, and gently disrupted. Levels of malondialdehyde (MDA), DNA fragmentation (DNAF), glutathione (GSH), nitrates/nitrites (NOx), superoxide dismutase (SOD), catalase (CAT) were determined using standard assays. Sperm maturity and deformation (DFO) was quantified microscopically. Results: Overall, the herb treatments decreased MDA levels by (60e90%), increased SOD activity (15e50%), and decreased DNAF (6e11%). Treatments with basil and oregano at 0.1% (w/v) were most effective. Conclusion: Our suggests that phytochemicals from Lamiaceae herbs modulate redox stress response, and protect against oxidative stress in seminal vesicles.

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      Vattem, D. A., & DeLeon, R. C. (). Modulation of redox homeostasis by Lamiaceae herbs in seminal vesicles of Lumbricus terrestris. Free Radicals and Antioxidants, 3(2), 93–99. https://doi.org/10.1016/j.fra.2013.05.003