Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Free Radicals and AntioxidantsNOW IN
    Article

    Chemical composition and antioxidant activity of the ethanol extract and purified fractions of cadillo (Pavonia sepioides)

    Cristian A. Gasca1★, Fabio A. Cabezas1, Laura Torras2, Jaume Bastida2, Carles Codina2★ Corresponding author

    1. 1Departamento de Química, Grupo de Química de Compuestos Bioactivos, Universidad del Cauca, Popayán A.A. 304, COLOMBIA.
    2. 2Departament de Productes Naturals, Facultat de Farmácia, Universitat de Barcelona, Barcelona 08028, SPAIN.

    CORRESPONDENCE

    Cristian A. Gasca

    Departamento de Química, Grupo de Química de Compuestos Bioactivos, Universidad del Cauca, Popayán A.A. 304, COLOMBIA.

    cgasca@unicauca.edu.co, gsc1061@hotmail.com

    Volume 3, Issue 2S · pp. S55–S61 · PUBLISHED · DOI: 10.1016/j.fra.2013.09.001

    View on Free Rad. Antiox. original site ↗

    ABSTRACT

    Background: Bioactive plant compounds can contribute to prevent diseases related to oxidative stress. Some of these compounds are water soluble and fat soluble vitamins, carotenoids and a huge variety of phenolic compounds associated with the antioxidant activity (AOA). Objective: The aim of this study was to study the antioxidant activity of Pavonia sepioides. Methods:The AOA was evaluated by spectrophotometric methods using the free radical DPPH* and the ABTS*þ radical cation assays. Total phenolic content (TPC) was determined by FolineCiocalteu method and total flavonoid content (TFC) was determined by direct reaction with aluminum trichloride. The phenolic content was evaluated by GC/MS technique. Results:The greatest antioxidant activity was found in ethyl acetate fraction (Fea), The total ethanol extract (TE) showed good AOA in comparison with hexane fraction (Fhx), dichloromethane fraction (Fdc) and aqueous fraction (Faq). The TPC and TFC analyses provided results consistent with the AOA with highest values for Fea and ET respectively. Different phenolic compounds of low molecular weight were identified by GC/MS (Salicylic acid, cinnamic acid, p-hydroxybenzoic acid, p-hydroxyphenylacetic acid, phydrocinnamic acid, vanillic acid, gentisic acid, p-cinnamic acid, o-Cinnamic acid, protocatechuic acid, syringic acid, m-cinnamic acid, ferulic acid, and caffeic acid). These compounds could be associated with the named activity. The Pearson and Spearman association tests indicated a positive linear correlation between the AOA and TPC and TFC results, showing correlation coefficients greater than 0.810 in the Pearson test and greater than 0.700 in the Spearman test (p < 0.05). Conclusion: The AAO of P. sepioides (ET and purified fractions) was confirmed. The study provides evidence that P. sepioides is potential source of natural antioxidants showing positive correlations between phenolic contents and the AAO.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Gasca, C. A., Cabezas, F. A., Torras, L., Bastida, J., & Codina, C. (). Chemical composition and antioxidant activity of the ethanol extract and purified fractions of cadillo (Pavonia sepioides). Free Radicals and Antioxidants, 3(2S), S55–S61. https://doi.org/10.1016/j.fra.2013.09.001