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    Wound Healing Activity and Chemical Standardization of Eugenia pruniformis Cambess

    Ricardo Diego Duarte Galhardo de Albuquerque1,2, Jamila Alessandra Perini3, Daniel Escorsim Machado3, Thaís Angeli-Gamba3, Ricardo dos Santos Esteves2, Marcelo Guerra Santos4, Adriana Passos Oliveira5★, Leandro Rocha1,2★ Corresponding author

    1. 1Programa de Pós Graduação em Biotecnologia Vegetal, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
    2. 2Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brasil.
    3. 3Laboratório de Pesquisas em Ciências Farmacêuticas, Unidade de Farmácia, Centro Universitário Estadual da Zona Oeste do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
    4. 4Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, São Gonçalo, RJ, Brasil.
    5. 5Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.

    CORRESPONDENCE

    Adriana Passos Oliveira

    Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.

    adrianapassos@pharma.ufrj.br

    Received: 12-12-2015; Revised: 03-02-2016; Accepted: 03-02-2016.

    Volume 12, Issue 48 · pp. 288–94 · PUBLISHED 13 October 2016 · DOI: 10.4103/0973-1296.192206

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Eugenia pruniformis is an endemic species from Brazil. Eugenia genus has flavonoids as one of the remarkable chemical classes which are related to the improvement of the healing process. Aims: To evaluate of wound healing activity of E. pruniformis leaves and to identify and quantify its main flavonoids compounds. Materials And Methods: Wound excision model in rats was used to verify the hydroethanolic and ethyl acetate extracts potential. The animals were divided in four groups of six and the samples were evaluated until the 15º day of treatment. Hydroxyproline dosage and histological staining with hematoxilin-eosin and Sirius Red were used to observe the tissue organization and quantify the collagen deposition, respectively. Chemical compounds of the ethyl acetate extract were identified by chromatographic techniques and mass spectrometry analysis and total flavonoids content was determined by spectrophotometric method. The antioxidant activity was determined by oxygen radical absorbing capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazylhydrate radical photometric (DPPH) assays. Results: The treated group with the ethyl acetate extract showed collagen deposition increase, higher levels of hidroxyproline, better tissue reorganization and complete remodeling of epidermis. Quercetin, kaempferol and hyperoside were identified as main compounds and flavonoids content value was 43% (w/w). The ORAC value of the ethyl acetate extract was 0.81± 0.05 mmol TE/g whereas the concentration to produce 50% reduction of the DPPH was 7.05± 0.09 µg/mL. Conclusion: The data indicate a wound healing and antioxidant activities of E. pruniformis. This study is the first report of flavonoids and wound healing activity of E. pruniformis.

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      Albuquerque, R. D. D. G. D., Perini, J. A., Machado, D. E., Angeli-Gamba, T., Esteves, R. D. S., Santos, M. G., Oliveira, A. P., & Rocha, L. (2016). Wound Healing Activity and Chemical Standardization of Eugenia pruniformis Cambess. Pharmacognosy Magazine, 12(48), 288–94. https://doi.org/10.4103/0973-1296.192206