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    Quantification Analysis and In Vitro Anti-Inflammatory Effects of 20-Hydroxyecdysone, Momordin Ic, and Oleanolic Acid from the Fructus of Kochia scoparia

    Sae-Rom Yoo1, Soo-Jin Jeong2, Na-Ri Lee2, Hyeun-Kyoo Shin2, Chang-Seob Seo2★★ Corresponding author

    1. 1K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon, Korea.
    2. 2KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea.

    CORRESPONDENCE

    Chang-Seob Seo

    KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea.

    csseo0914@kiom.re.kr

    Received: 05-10-2016; Revised: 11-07-2016; Accepted: 07-11-2016.

    Volume 13, Issue 51 · pp. 339–344 · PUBLISHED 19 July 2017 · DOI: 10.4103/0973-1296.211023

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The fructus of Kochia scoparia Schrader (Chenopodiaceae) is a traditional herbal medicine that has been used for treating gonorrhea and dermatitis. Objective: We investigated the anti-inflammatory activities of three marker compounds, including 20-hydroxyecdysone, momordin Ic, and oleanolic acid, from the fructus of K. scoparia. Materials and Methods: The simultaneous analysis of three components was performed using high-performance liquid chromatography and high-performance thin-layer chromatography. We evaluated the anti-inflammatory effects of the nine marker compounds by determining their anti-inflammatory activities in the murine macrophage cell line RAW 264.7. Results: Among three marker compounds, momordin Ic, but not 20-hydroxyecdysone and oleanolic acid, had inhibitory effects on the production of inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in LPS-treated RAW264.7 macrophages. The effects of three marker compounds on prostaglandin E2 (PGE2) were also evaluated. All three compounds significantly reduced PGE2 production in LPS-treated cells. Conclusions: We suggest that momordin Ic is the most potent phytochemical of the fructus of K. scoparia as an anti-inflammatory agent.

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      Yoo, S., Jeong, S., Lee, N., Shin, H., & Seo, C. (2017). Quantification Analysis and In Vitro Anti-Inflammatory Effects of 20-Hydroxyecdysone, Momordin Ic, and Oleanolic Acid from the Fructus of Kochia scoparia. Pharmacognosy Magazine, 13(51), 339–344. https://doi.org/10.4103/0973-1296.211023