Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Biological Activities and Cytotoxicity of Eperua oleifera Ducke Oil-resin

    Fernanda Torlania Alves Gomes1, Ana Paula de Araújo Boleti1, Lidiam M. Leandro2, Diego Squinello2, Ellen S. P. Aranha1, Marne C. Vasconcelos1, Paul Cos3, Valdir F. Veiga‑Junior2, Emerson Silva Lima1★★ Corresponding author

    1. 1Biological Activity Laboratory, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
    2. 2Chemistry Department, Exact Sciences Institute, Federal University of Amazonas, Manaus, Amazonas, Brazil.
    3. 3Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, B2610 Antwerp, Belgium.

    CORRESPONDENCE

    Emerson Silva Lima

    Biological Activity Laboratory, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.

    eslima@ufam.edu.br

    Received: 06-12-2016; Revised: 01-02-2017; Accepted: 01-02-2017.

    Volume 13, Issue 52 · pp. 542–52 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_552_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The oil‑resin of Eperua oleifera Ducke has been used in popular medicine similarly to the copaiba oil (Copaifera spp.). Objective: This study aimed to investigate the effects of the acid fraction of E. oleifera oil‑resin (AFEOR) on cell proliferation, collagen production in human fibroblasts, inhibition of metalloproteinases, and cytotoxicity against tumor cell lines. Materials and Methods: Acid fraction of E. oleifera was fractionated in the ion exchange column chromatography. Cytotoxicity and genotoxicity were evaluated by Alamar Blue® and Cometa assay. The inhibition of metalloproteinases was performed by zymography and Western blotting. Results: The predominant acidic diterpenes in the AFEOR were copalic and hardwickiic acids. AFEOR caused morphology alteration and decrease of proliferation at concentrations higher than 5 µg/mL. It also caused significant collagen proliferation in fibroblasts. It showed cytotoxicity against tumoral and nontumoral cell lines, with IC50 values ranging from 13 to 50 µg/mL, and a hemolytic activity with an IC50 value of 38.29 µg/mL. AFEOR inhibited collagenase activity, with an IC50 value of 46.64 µg/mL, and matrix metalloproteinase‑2 (MMP)‑2 and MMP‑9 in HaCaT cells or MMP‑1 expression in MRC‑5 cells. AFEOR induced genotoxicity in MRC‑5 cells with a DNA damage index between 40% and 60% when compared to the negative controls (0%–20%). Conclusion: For the first time, biological activities from oil‑resin E. oleifera demonstrated ratifying somehow its popular use.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Gomes, F. T. A., Boleti, A. P. D. A., Leandro, L. M., Squinello, D., Aranha, E. S. P., Vasconcelos, M. C., Cos, P., Veiga‑Junior, V. F., & Lima, E. S. (2017). Biological Activities and Cytotoxicity of Eperua oleifera Ducke Oil-resin. Pharmacognosy Magazine, 13(52), 542–52. https://doi.org/10.4103/pm.pm_552_16