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    Article

    Bioassay-guided Isolation of Neuroprotective Fatty Acids from Nigella sativa against 1-methyl-4-phenylpyridinium-induced Neurotoxicity

    Leila Hosseinzadeh1, Hoda Monaghash2, Farahnaz Ahmadi3, Nastaran Ghiasvand4, Yalda Shokoohinia5★★ Corresponding author

    1. 1Research Center of Oils and Fats, Kermanshah University of Medical Sciences, Kermanshah, Iran.
    2. 2Student Research Committee, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
    3. 3Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
    4. 4Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
    5. 5Department of Pharmacognosy and Biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

    CORRESPONDENCE

    Yalda Shokoohinia

    Department of Pharmacognosy and Biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

    yshokoohinia@kums.ac.ir

    Received: 21-10-2016; Revised: 02-12-2016; Accepted: 02-12-2016.

    Volume 13, Issue 52 · pp. 627–633 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_470_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: Parkinson’s disease, a slowly progressive neurological disease, is associated with degeneration of the basal ganglia of the brain and a deficiency of the neurotransmitter dopamine. The main aspects of researches are the protection of normal neurons against degeneration. Fatty acids (FAs), the key structural elements of dietary lipids, are carboxylic straight chains and notable parameters in nutritional and industrial usefulness of a plant. Materials and Methods: Black cumin, a popular anti‑inflammatory and antioxidant food seasoning, contains nonpolar constituents such as FAs which were extracted using hexane. Different fractions and subfractions were apt to cytoprotection against apoptosis and inflammation induced by 1‑methyl‑4‑phenylpyridinium (MPP+) in rat pheochromocytoma cell line (PC12) as a neural cell death model. The experiment consisted of examination of cell viability assessment, mitochondrial membrane potential (MMP), caspase‑3 and ‑9 activity, and measurement of cyclooxygenase (COX) activity. Results: MPP+ induced neurotoxicity in PC12 cells. Pretreatment with subfractions containing FA mixtures attenuated MPP+‑mediated apoptosis partially dependent on the inhibition of caspase‑3 and ‑9 activity and increasing the MMP. A mixture of linoleic acid, oleic acid, and palmitic acid also decreased the COX activity induced by MPP+ in PC12 cells. Conclusion: Our observation indicated that subtoxic concentration of FA from Nigella sativa may exert cytoprotective effects through their anti‑apoptotic and anti‑inflammation actions and could be regarded as a dietary supplement.

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      Hosseinzadeh, L., Monaghash, H., Ahmadi, F., Ghiasvand, N., & Shokoohinia, Y. (2017). Bioassay-guided Isolation of Neuroprotective Fatty Acids from Nigella sativa against 1-methyl-4-phenylpyridinium-induced Neurotoxicity. Pharmacognosy Magazine, 13(52), 627–633. https://doi.org/10.4103/pm.pm_470_16