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    Article

    Rapid Quantitative Analysis of Naringenin in the Fruit Bodies of Inonotus vaninii by Two-phase Acid Hydrolysis Followed by Reversed Phase-high Performance Liquid Chromatography-ultra Violet

    Xia Guohua1, Ruirong Pan2, Rui Bao3, Yanru Ge3, Cunshan Zhou3★, Yuping Shen3★ Corresponding author

    1. 1Department of Food Sciences, School of Food Sciences and Engineering, Jiangsu University, Zhenjiang, Jiangsu, P.R. China.
    2. 2Department of Chinese Materia Medica and Pharmacy, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, P.R. China.
    3. 3Department of Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, P.R. China.

    CORRESPONDENCE

    Cunshan Zhou

    Department of Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, P.R. China.

    zhcs@ujs.edu.cn

    Received: 28-07-2016; Revised: 20-09-2016; Accepted: 13-11-2017.

    Volume 13, Issue 52 · pp. 659–662 · PUBLISHED 13 November 2017 · DOI: 10.4103/pm.pm_350_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Introduction: Sanghuang is one of mystical traditional Chinese medicines recorded earliest 2000 years ago, that included various fungi of Inonotus genus and was well-known for antitumor effect in modern medicine. Inonotus vaninii is grown in natural forest of Northeastern China merely and used as Sanghuang commercially, but it has no quality control specification until now. This study was to establish a rapid method of two-phase acid hydrolysis followed by reversed phase-high performance liquid chromatography-ultra violet (RP-HPLC-UV) to quantify naringenin in the fruit body of I. vaninii. Materials and Methods: Sample solution was prepared by pretreatment of raw material in two-phase acid hydrolysis and the hydrolysis technology was optimized. After reconstitution, analysis was performed using RP-HPLC-UV. The method validation was investigated and the naringenin content of sample and comparison were determined. Results: The naringenin was obtained by two-phase acid hydrolysis method, namely, 10.0 g of raw material was hydrolyzed in 200 mL of 1% sulfuric acid aqueous solution (v/v) and 400 mL of chloroform in oil bath at 110°C for 2 h. Good linearity (r = 0.9992) was achieved between concentration of analyte and peak area. The relative standard deviation (RSD) of precision was 2.47% and the RSD of naringenin contents for repeatability was 3.13%. The accuracy was supported with recoveries at 96.37%, 97.30%, and 99.31%. The sample solution prepared using the proposed method contained higher content of naringenin than conventional method and was stable for 8 h. Conclusion: Due to the high efficiency of sample preparation and high reliability of the HPLC method, it is feasible to use this method for routine analysis of naringenin in the fungus.

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      Guohua, X., Pan, R., Bao, R., Ge, Y., Zhou, C., & Shen, Y. (2017). Rapid Quantitative Analysis of Naringenin in the Fruit Bodies of Inonotus vaninii by Two-phase Acid Hydrolysis Followed by Reversed Phase-high Performance Liquid Chromatography-ultra Violet. Pharmacognosy Magazine, 13(52), 659–662. https://doi.org/10.4103/pm.pm_350_16