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    Article

    Rosmarinic Acid Inhibits Stem‑Like Breast Cancer through Hedgehog and Bcl‑2/Bax Signaling Pathways

    Hong Li1★, Yuefeng Zhang2, Hsiao‑Huei Chen3, En Huang3, Hailin Zhuang1, Dan Li1, Feng Ni2★★ Corresponding author

    1. 1Department of Health Management, Fujian Health College.
    2. 2Medical Technology Center, Fujian Health College, Fuzhou, Fujian, China.
    3. 3Department of Medicine, Brain and Mind Institute, University of Ottawa, Ottawa, Canada.

    CORRESPONDENCE

    Hong Li

    Department of Health Management, Fujian Health College.

    kinglh2002@126.com

    Received: 24-01-2019; Revised: 06-03-2019.

    Volume 15, Issue 65 · pp. 600–606 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_22_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: This study aimed to detect whether RA could inhibit the proliferation and migration of BCSCs through hedgehog (Hh) signaling while promoting apoptosis via Bcl‑2/ Bax. Materials and Methods: BCSCs from the human breast cancer cell line MD‑MB‑231 were isolated by fluorescence‑activated cell sorting with the surface markers of CD44+/CD24‑/low. The viability, migration, and apoptosis of BCSCs were assessed by the CCK‑8 assay, cell wound healing test, and flow cytometry for positive staining for Annexin V‑FITC and propidium iodine (PI), respectively. mRNA and protein levels of Hh and Bcl‑2/Bax signaling pathways were obtained by real‑time reverse transcriptase polymerase chain reaction and immunoblots. Results: RA inhibited the viability and migration of BCSCs and increased the numbers of late apoptotic cells. Consistent with the increased apoptosis, RA treatment downregulated Bcl‑2 while upregulating Bax expression. In line with its effect to limit migration, RA treatment inhibited the expression of Hh‑related genes smoothened and glioma‑associated oncogene homolog 1. Conclusion: The present study suggests that RA exerts anti‑cancer effects on BCSCs by inhibiting Bcl‑2 and Hh signaling pathways.

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      Li, H., Zhang, Y., Chen, H., Huang, E., Zhuang, H., Li, D., & Ni, F. (2019). Rosmarinic Acid Inhibits Stem‑Like Breast Cancer through Hedgehog and Bcl‑2/Bax Signaling Pathways. Pharmacognosy Magazine, 15(65), 600–606. https://doi.org/10.4103/pm.pm_22_19