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    Development of a Species‑Specific Polymerase Chain Reaction‑Based Technology for Authentication of Asini Corii Colla and Taurus Corii Colla

    Liqun Chen1, Zhaoqun Jiao1, Yufei Chen1, Pingtian Yu1, Yang Zheng1, Yaya Yang1, Yuping Shen1, Huan Yang1★★ Corresponding author

    1. 1Department of Chinese Medicine and Pharmacy, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu Province, China.

    CORRESPONDENCE

    Huan Yang

    Department of Chinese Medicine and Pharmacy, School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu Province, China.

    yanghuan1980@ujs.edu.cn

    Received: 21-12-2018; Revised: 14-01-2019.

    Volume 15, Issue 65 · pp. 607–612 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_640_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: In this study, we aimed to develop a species‑specific polymerase chain reaction (PCR) approach to detect deoxyribonucleic acid (DNA) fragments of ACC and TCC for rapid authentication purpose. Materials and Methods: Four sets of novel species‑specific primer were designed, and PCR conditions were optimized for the PCR assay, which was further validated for specificity and sensitivity. Then, deliberate mixture was analyzed to further verify the capability of adulteration detection by the developed PCR method. Finally, it was used to assess the authenticity of commercial products. Results: Four primers’ sets were specific for amplification of extracted mitochondrial DNA of donkey, bovine, swine, and horse when the annealing temperature was 59°C, 59°C, 63°C, and 65°C, and 40 thermal cycles was performed. Under this optimized PCR conditions, 1 ng/μL of horse DNA template has been detected in the sensitivity test, with 0.1 ng/μL for donkey, bovine, and swine. The assay was capable of detecting spiked species at 10% level in premixed ACC or TCC samples. Conclusion: The newly developed PCR‑based technology was specific and sensitive, and it was a convenient approach for the authentication of commercial ACC and TCC products.

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      Chen, L., Jiao, Z., Chen, Y., Yu, P., Zheng, Y., Yang, Y., Shen, Y., & Yang, H. (2019). Development of a Species‑Specific Polymerase Chain Reaction‑Based Technology for Authentication of Asini Corii Colla and Taurus Corii Colla. Pharmacognosy Magazine, 15(65), 607–612. https://doi.org/10.4103/pm.pm_640_18