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    Article

    In vitro and in vivo Anti-inflammatory Activity of the Methanolic Extract of the Leaves of Cissus quadrangularis

    Alaa El‑Din E. Abdel‑Hamid1, Riham Salah El Dine1, Jandirk Sendker2, Soheir M. El Zalabani1, Meselhy R. Meselhy1, Elena Jimenez‑Negro2, Ashraf B. Abdel‑Naim3★★ Corresponding author

    1. 1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    2. 2Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany.
    3. 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

    CORRESPONDENCE

    Ashraf B. Abdel‑Naim

    Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

    abnaim@yahoo.com

    Received: 26-10-2018; Revised: 05-12-2018.

    Volume 15, Issue 65 · pp. 659–670 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_547_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: To assess the liver antifibrotic action of ethanolic extract of aerial parts of Solanum villosum Mill subsp. miniatum (Bernh. ex Willd.) (SVE) and correlate this activity with its high performance liquid chromatography‑quadrupole time of flight (HPLC‑qTOF) Electrospray Ionisation Mass Spectrometry (ESIMS) phytochemical profile. Materials and Methods: The median lethal dose of SVE was determined, and a rat model of carbon tetrachloride (CCl4)‑induced liver fibrosis was used to evaluate its antifibrotic activity. Markers for hepatotoxicity, fibrosis, and oxidative stress were assessed, and histopathological features of liver tissues were examined. Metabolite profiling of SVE was achieved via HPLC‑qTOF‑ESIMS coupled with Photodiode array (PDA). Liver fibrosis was induced in rats by oral administration of CCl4 for 6 weeks. Silymarin (positive control) and SVE (100 and 250 mg/kg) were orally administered daily for 6 weeks. Results: Compared to CCl4‑intoxicated group, administration of SVE obviously ameliorated fibrosis of the hepatic capsule associated with aggregation of multiple focal fat cells formation. Both silymarin and SVE ameliorated the rise in serum markers of hepatotoxicity (alanine transaminase, aspartate transaminase, and alkaline phosphatase), markedly attenuated CCl4‑induced oxidative stress. The antifibrotic activity of SVE was evidenced by inhibiting the rise in hepatic hydroxyproline content and accumulation of collagen. This was confirmed by the ability of SVE to inhibit alterations in expression of the fibrosis‑related genes Collagen Iα, matrixmetalloproteinase‑2 (MMP‑2), tissue inhibitor matrix metalloproteinase‑2, and transforming growth factor beta 1. HPLC‑qTOF‑ESIMS analysis of SVE revealed the presence of 47 metabolites, among which 33 were tentatively identified. Conclusion: SVE exhibited hepatoprotective and antifibrotic activities in rats by enhancing the antioxidant capacity and regulating expression of fibrogenic mediators.

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      Abdel‑Hamid, A. E. E., Dine, R. S. E., Sendker, J., Zalabani, S. M. E., Meselhy, M. R., Jimenez‑Negro, E., & Abdel‑Naim, A. B. (2019). In vitro and in vivo Anti-inflammatory Activity of the Methanolic Extract of the Leaves of Cissus quadrangularis. Pharmacognosy Magazine, 15(65), 659–670. https://doi.org/10.4103/pm.pm_547_18