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    Renoprotective Effects of the Hydroethanolic Extract of Senecio serratuloides against Nw‑Nitro L‑arginine Methyl Ester‑induced Oxidative Stress in Wistar Rats

    Charlotte Mungho Tata2,3, Constance Rufaro Sewani‑Rusike2, Opeoluwa Oyehan Oyedeji4, Ephraim Tobela Gwebu5, Benedicta Ngwenchi Nkeh‑Chungag6★★ Corresponding author

    1. 1Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha, South Africa.
    2. 2Department of Chemical Sciences, Faculty of Science, University of Johannesburg, Johannesburg, South Africa.
    3. 3Department of Chemistry, Faculty of Science and Agriculture, University of Fort Hare, Eastern Cape Province, South Africa.
    4. 4Department of Chemistry, Faculty of Science and Technology, Rusangu University, Monze, Zambia, South Africa.
    5. 5Department of Biological Sciences, Faculty of Natural Sciences, Walter Sisulu University, Mthatha, South Africa.

    CORRESPONDENCE

    Benedicta Ngwenchi Nkeh‑Chungag

    bnkehchungag@wsu.ac.za

    Received: 21-08-2019; Revised: 13-11-2019; Accepted: 21-04-2020.

    Volume 16, Issue 70S · pp. S418–S424 · PUBLISHED 28 August 2020 · DOI: 10.4103/pm.pm_375_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study was aimed at investigating the renoprotective and antioxidant capacity of hydroethanolic extract of Senecio serratuloides (HESS). Materials and Methods: In vitro and ex vivo antioxidant capacity of the extract was investigated. Female Wistar rats were treated with Nw‑nitro L‑arginine methyl ester (L‑NAME) (40 mg/kg) for 4 weeks and then cotreating with L‑NAME (20 mg/kg) and extract (HESS150 or HESS300 mg/kg) for 2 weeks and finally with plant extract or normal saline only for 2 weeks making a total of 8 weeks. Twenty‑four‑hour urine samples were collected during the study, and at the end of the study; blood and kidneys were harvested for biochemical and histological assays. Results: HESS exhibited high antiradical activity against 1,1‑diphenyl‑2‑picrylhydrazyl and 2,2’‑azinobis (3‑ethylbenzothiazoline‑6‑sulfonic acid) radicals with IC50 values of 0.1 and 0.4 mg/ml, respectively. HESS significantly (P < 0.01) augmented L‑NAME‑induced decrease in creatinine clearance, glomerular filtration rate, and serum calcium concentration. HESS significantly increased in vivo antioxidant capacity (P < 0.01), decreased malondialdehyde (P < 0.01), and Bax (P < 0.001) concentration. It showed renoprotection and significantly (P < 0.01) prevented collagen deposition in the kidneys. Conclusion: S. serratuloides has renoprotective and free radical‑scavenging properties and therefore maybe important in combating oxidative stress‑mediated diseases in the kidneys and other parts of the body.

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      Tata, C. M., Sewani‑Rusike, C. R., Oyedeji, O. O., Gwebu, E. T., & Nkeh‑Chungag, B. N. (2020). Renoprotective Effects of the Hydroethanolic Extract of Senecio serratuloides against Nw‑Nitro L‑arginine Methyl Ester‑induced Oxidative Stress in Wistar Rats. Pharmacognosy Magazine, 16(70S), S418–S424. https://doi.org/10.4103/pm.pm_375_19