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    Article

    Protective Mechanisms of Piperine against Renal Ischemia– Reperfusion Injury in Rats

    Mohamed Aly Morsy2,3★, Mahmoud El‑Daly4, Anwaar S. M. Abu Shnaf5, Sherif W. Mansour6, Ahmed R. N. Ibrahim7,8★ Corresponding author

    1. 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al‑Ahsa, Saudi Arabia.
    2. 2Department of Pharmacology, Faculty of Medicine, Minia University.
    3. 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University.
    4. 4Department of Zoology, Faculty of Science, Minia University, El‑Minia.
    5. 5Department of Physiology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
    6. 6Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia.
    7. 7Department of Biochemistry, Faculty of Pharmacy, Minia University, El‑Minia, Egypt.

    CORRESPONDENCE

    Mohamed Aly Morsy

    Department of Pharmacology, Faculty of Medicine, Minia University.

    momorsy@kfu.edu.sa

    Received: 10-01-2020; Revised: 26-02-2020; Accepted: 21-04-2020.

    Volume 16, Issue 70S · pp. S425–S430 · PUBLISHED 28 August 2020 · DOI: 10.4103/pm.pm_586_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: We hypothesized that piperine would protect against renal IRI in rats via inhibition of oxidative stress and inflammation. Materials and Methods: Male Sprague Dawley rats were subdivided into four groups; sham, IR, IR + piperine, and sham + piperine. All animals have been treated for 4 days with either vehicle or piperine (100 mg/kg/day). One hour after the last piperine or vehicle administration, animals were subjected to bilateral renal ischemia for 45 min by clamping both renal pedicles, followed by reperfusion for 24 h. At the end of the experiments, kidneys were harvested for the determination of lipid peroxidation (malondialdehyde [MDA]), reduced glutathione (GSH), inflammatory and apoptotic markers, and histopathology. Serum levels of creatinine and urea have been determined. Results: Induction of renal IR increased renal oxidative stress (increased MDA and decreased GSH) and the expression levels of inflammatory and proapoptotic genes (nuclear factor‑kappa B, inducible nitric oxide synthase, cyclooxygenase‑2, and caspase‑3). Moreover, serum levels of creatinine and urea were significantly elevated. Alternatively, pretreatment of the animals with piperine resulted in normalization of these parameters. Conclusion: The results showed that piperine pretreatment protects against IRI in rat kidneys via mechanisms involving amelioration of oxidative stress along with inflammatory and apoptotic pathways.

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      Morsy, M. A., El‑Daly, M., Shnaf, A. S. M. A., Mansour, S. W., & Ibrahim, A. R. N. (2020). Protective Mechanisms of Piperine against Renal Ischemia– Reperfusion Injury in Rats. Pharmacognosy Magazine, 16(70S), S425–S430. https://doi.org/10.4103/pm.pm_586_19