Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Honey as a Solvent for the Green Extraction, Analysis, and Bioconversion of Daidzin from Pueraria candollei var. mirifica Root

    Suppalak Phaisan2, Gorawit Yusakul2★, Poomraphie Nuntawong3, Seiichi Sakamoto3, Waraporn Putalun4,5, Satoshi Morimoto3, Hiroyuki Tanaka6★ Corresponding author

    1. 1Department of Industrial Pharmacy, School of Pharmacy, Walailak University.
    2. 2Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812‑8582, Japan.
    3. 3Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University.
    4. 4Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology, National Research University‑Khon Kaen University, Khon Kaen 40002, Thailand.
    5. 5Department of Pharmacognosy and Kampo, Faculty of Pharmaceutical Sciences, Sanyo‑Onoda City University, Yamaguchi, Japan.

    CORRESPONDENCE

    Gorawit Yusakul

    Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812‑8582, Japan.

    gorawit.yu@wu.ac.th

    Received: 27-02-2020; Revised: 09-04-2020; Accepted: 15-07-2020.

    Volume 16, Issue 71 · pp. 524–530 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_74_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study aims to apply honey (H‑NADES) and a sugar‑based NADES (S‑NADES) for the extraction, analysis, and bioconversion of daidzin from Pueraria candollei var. mirifica (PM) root. Materials and Methods: Various concentrations of H‑NADES and S‑NADES (water:sucrose:glucose: fructose, 18:3:18:22 by weight) were used as solvents for extraction. Indirect competitive enzyme‑linked immunosorbent assay (icELISA) was developed and validated for monitoring the extraction efficacy. The catalytic reactivity against daidzin of β‑glucosidase purified from honey was investigated. Results: Using NADESs as solvents, icELISA was suitable for the reliable determination of daidzin with high sensitivity (1.95–125 ng/mL), specificity (% cross‑reactivity ≤ 2.60), and accuracy (98.3‑106% daidzin recovery). Daidzin at a concentration of 75.8 ± 3.67 µg/mL was extracted using 50% (v/v) S‑NADES, which was the most effective for the extraction compared to H‑NADES, water and ethanol. In addition, daidzin was converted to daidzein by honey β‑glucosidase. Conclusion: Both S‑NADES and H‑NADES were useful for the extraction, analysis, and bioconversion of daidzin, and β‑glucosidase from honey might enhance the oestrogenic activity and bioavailability of PM phytochemicals.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Phaisan, S., Yusakul, G., Nuntawong, P., Sakamoto, S., Putalun, W., Morimoto, S., & Tanaka, H. (2020). Honey as a Solvent for the Green Extraction, Analysis, and Bioconversion of Daidzin from Pueraria candollei var. mirifica Root. Pharmacognosy Magazine, 16(71), 524–530. https://doi.org/10.4103/pm.pm_74_20