Isovitexin, A New Metabolite, Was Found in the Metabolites of Co-cultured Five Flavonoids Isolated from Ziziphus jujuba Mill var. spinosa Seeds by Rat Intestinal Flora
Ying Liu1, Junbo Xie2, Xusheng Cui3,4, Yanqing Zhang1★, Kunsheng Zhang1★★ Corresponding author
- 1Department of Food Science, College of Biotechnology and Food Science, Tianjin University of Commerce, China.
- 2Department of Chinese Materia Medica, School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 2 Department of Medicinal Resources, Shijiazhuang Yiling Pharmaceutical Co. Ltd., Hebei, 3 Department of Chinese Medicinal Materials Research Center, China Agricultural University, Beijing, China.
CORRESPONDENCE
Yanqing Zhang
Department of Food Science, College of Biotechnology and Food Science, Tianjin University of Commerce, China.
Received: 20-10-2019; Revised: 04-03-2020; Accepted: 27-05-2020.
Volume 16, Issue 71 · pp. 557–563 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_454_19
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ABSTRACT
Objectives: The primary objective of this study was to investigate the mechanism of degradation of co‑cultured flavonoids, namely 6´´´‑feruloyl spinosin, 6´´´‑p‑coumaroyl spinosin, spinosin, swertisin, and isovitexin, in the intestinal flora of rats under in vitro conditions. Materials and Methods: In this study, we co‑cultured the five flavonoids in the intestinal flora of rats under in vitro conditions and determined the degradation of these five flavonoids via high‑performance liquid chromatography with tandem mass spectrometry (MS/MS). Results: The degradation rate of the 6´´´‑feruloyl spinosin, 6´´´‑p‑coumaroyl spinosin, and swertisin was affected by the concentration of the sample and conforms to the first‑order kinetic model. According to our results, the 6´´´‑feruloyl spinosin and 6´´´‑p‑coumaroyl spinosin may be degraded to spinosin and swertisin, and spinosin continued to decompose into swertisin. These results were consistent with their individual experiments. Moreover, by comparing the structures of isovitexin and swertisin, isovitexin was first found to be the product of the seventh demethylation reaction of the flavonoid core structure, demonstrating that isovitexin is a metabolite of ZSS flavonoids following spinosin and swertisin. Conclusion: Taken together, the results of this study explain the metabolic and interrelation of these five main flavonoids in vitro.
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Liu, Y., Xie, J., Cui, X., Zhang, Y., & Zhang, K. (2020). Isovitexin, A New Metabolite, Was Found in the Metabolites of Co-cultured Five Flavonoids Isolated from Ziziphus jujuba Mill var. spinosa Seeds by Rat Intestinal Flora. Pharmacognosy Magazine, 16(71), 557–563. https://doi.org/10.4103/pm.pm_454_19