Fisetin Attenuates Gastric Mucosal Lesions through Modulating Nuclear Factor‑Kappa B and Peroxisome Proliferator‑Activated Receptor‑γ in Rats
Jinchen Hu1, Li Cai2, Zengwu Yao1, Zhenbin Zhang1, Menglai Zhang1, Yifei Zhang1, Lixin Jiang1, Baohong Hu3★★ Corresponding author
- 1Departments of Gastrointestinal Surgery, Hospital of Qingdao University, Yantai, China.
- 2Pathology and, China.
- 3Medical Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
CORRESPONDENCE
Baohong Hu
Medical Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China.
Received: 09-01-2020; Revised: 26-02-2020; Accepted: 21-04-2020.
Volume 16, Issue 71 · pp. 605–612 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_4_20
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Objective: The objective of this study was to explore the underlying mechanism of action of fisetin on ethanol‑induced gastric ulcer model. Materials and Methods: In this study, gastric mucosal lesions were induced by ethanol in rats. Five groups of rats were formed based on the treatment administered: model group (model), omeprazole (40 mg/kg) group (omeprazole), high‑dose fisetin group (100 mg/kg, H‑fisetin), medium‑dose fisetin group (50 mg/kg, M‑fisetin), and low‑dose fisetin group (25 mg/kg, L‑fisetin). Interleukin (IL)‑1β, IL‑6, and tumor necrosis factor (TNF)‑α levels were assessed in serum. The expression of peroxisome proliferator‑activated receptor (PPAR)‑γ, nuclear factor‑kappa B (NF‑κB), and p38‑mitogen‑activated protein kinase (p38‑MAPK) in the gastric mucosa was also measured. Results: In the case of the high‑dose fisetin group, the level of TNF‑α, IL‑1β, and IL‑6 decreased from 9.57 pg/mL to 5.19 pg/mL, from 0.59 pg/mL to 0.27 pg/mL, and from 37.96 pg/mL to 21.09 pg/mL, respectively. In the case of the omeprazole group, the level of TNF‑α, IL‑1β, and IL‑6 decreased to 4.38 pg/mL, 0.27 pg/mL, and 18.58 pg/mL, respectively. The expression of PPAR‑γ protein in the high‑dose fisetin and omeprazole groups was about 1.5 times higher than that in the model group. Compared with the model group, the expression of NF‑κB protein reduced to 0.34 level and 0.47 level in the omeprazole and high‑dose fisetin groups, respectively. Compared with the model group, the expression of p38‑MAPK protein reduced to 0.55 level and 0.68 level in the omeprazole and high‑dose fisetin groups, respectively. Conclusion: Fisetin might relieve the symptoms of ethanol‑induced gastric ulcer in rats through the regulation of NF‑κB pathway.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Hu, J., Cai, L., Yao, Z., Zhang, Z., Zhang, M., Zhang, Y., Jiang, L., & Hu, B. (2020). Fisetin Attenuates Gastric Mucosal Lesions through Modulating Nuclear Factor‑Kappa B and Peroxisome Proliferator‑Activated Receptor‑γ in Rats. Pharmacognosy Magazine, 16(71), 605–612. https://doi.org/10.4103/pm.pm_4_20