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    Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail

    Yuh‑Ming Chang2,3★, Ming‑Cheng Lin4,5★, Ling‑Zong Hong6, Pei‑Ni Chen3, Yu‑Hsin Tsai3, You‑Cheng Hseu7,8,9, Ke‑Min Chen10, Li‑Sung Hsu3,11★, Ting‑Hsien Kao12,13★★ Corresponding author

    1. 1Department of Neurology, Division of Internal Medicine, Hsinchu Mackay Memorial Hospital, Hsinchu, Taiwan.
    2. 2Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taiwan.
    3. 3Department of Internal Medicine, Chung Shan Medical University Hospital, Taiwan.
    4. 4School of Medicine, Chung Shan Medical University, Taiwan.
    5. 5Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
    6. 6Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taiwan.
    7. 7Department of Health and Nutrition Biotechnology, Asia University, Taiwan.
    8. 8Chinese Medicine Research Center, China Medical University, Taiwan.
    9. 9Department of Parasitology, School of Medicine, Chung Shan Medical University, Taiwan.
    10. 10Clinical Laboratory, Chung Shan Medical University Hospital, Taiwan.
    11. 11Division of Functional Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taiwan.
    12. 12Department of Neurosurgery, Lin Shin Medical Corporation Lin Shin Hospital, Taichung, Taiwan.

    CORRESPONDENCE

    Yuh‑Ming Chang

    Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taiwan.

    Ishsu405@yahoo.com.tw

    Received: 19-01-2020; Revised: 09-04-2020; Accepted: 06-05-2020.

    Volume 16, Issue 71 · pp. 620–624 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_31_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: In this study, we investigate the effects of naringenin on osteosarcoma. Materials and Methods: 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetra zolium bromide (MTT) assay was performed to detect the cell viability. Zymography assay and transwell assay were used to measure the matrix metalloproteinase (MMP) activity and migration ability. Western blot analysis was used to determine the expression of migration‑related proteins. Results: No overt alternation of cytotoxicity in response to different concentrations of naringenin for 24 h was found by MTT assay. MMP‑2 and MMP‑9 activities and expression were significantly repressed by naringenin in a dose‑dependent manner, as evidenced by zymography and Western blot analysis. Naringenin dose dependently reduced the expression of mesenchymal marker Snail. Conclusion: These results indicate that naringenin exhibited antimigration property through inhibition of MMP‑2 and MMP‑9 and downregulation of Snail expression. Thus, naringenin may be a potential inhibitor of metastasis of osteosarcoma.

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      Chang, Y., Lin, M., Hong, L., Chen, P., Tsai, Y., Hseu, Y., Chen, K., Hsu, L., & Kao, T. (2020). Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail. Pharmacognosy Magazine, 16(71), 620–624. https://doi.org/10.4103/pm.pm_31_20