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    Genotoxic Assay of Silver and Zinc Oxide Nanoparticles Synthesized by Leaf Extract of Garcinia livingstonei T. Anderson: A Comparative Study

    Azharuddin B. Daphedar1, Siddappa B. Kakkalameli2, Govindappa Melappa2, Tarikere Chandrashekharappa Taranath3, Chandrashekar Srinivasa4, Chandan Shivamallu5, Asad Syed6, Najat Marraiki6, Abdallah M. Elgorban6, Ravindra Veerapur7, Sharangouda S Patil8, Shiva Prasad Kollur9★★ Corresponding author

    1. 1Anjuman Arts, Science and Commerce College, Vijayapura.
    2. 2Department of Botany, Davangere University, Shivagangotri, Davangere.
    3. 3Environmental Biology Laboratory, P. G. Department of Studies in Botany, Karnatak University, Dharwad.
    4. 4Department of Studies in Biotechnology, Davangere University, Shivagangotri, Davangere.
    5. 5Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysore, Karnataka, India.
    6. 6Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
    7. 7Department of Metallurgy and Materials Engineering, Malawi Institute of Technology, Malawi University of Science and Technology, Limbe, Malawi.
    8. 8ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Yelahanka, Bengaluru.
    9. 9Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka, India.

    CORRESPONDENCE

    Shiva Prasad Kollur

    Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka, India.

    shivachemist@gmail.com

    Received: 30-12-2020; Revised: 23-02-2021; Accepted: 27-03-2021.

    Volume 17, Issue 5S · pp. S114–S121 · PUBLISHED 10 June 2021 · DOI: 10.4103/pm.pm_536_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The as‑prepared NPs were characterized by ultraviolet‑visible spectroscopy, infrared spectroscopy (FT‑IR), X‑ray diffraction (XRD), atomic force microscopy (AFM), and high‑resolution transmission electron microscopy (HR‑TEM). Analysis of FT‑IR spectrum revealed that certain functional groups behaved as reducing and stabilizing agents in the formation of nanostructures. The crystalline nature of the AgNPs and ZnONPs was confirmed by XRD analysis. The size and shape of the as‑obtained materials were found using HR‑TEM analysis and were in the range of 5–65 nm and 38–94 nm for AgNPs and ZnONPs, respectively. Further, the root cells of C. arietinum were treated with both AgNPs and ZnONPs in different concentrations (5, 25, 50, and 100 µg/ml) for 24 h at the interval of 3, 6, 12, and 24 h along with distilled water as control. Results: The study clearly indicated that the AgNPs and ZnONPs showed an inhibitory effect on the cell division in root tip cells and caused a decrease in their mitotic index (MI) values. The reduction in MI in AgNPs is more evident than that of ZnONPs when compared to control. Aberrations in chromosomal behavior such as micronucleus, sticky chromosomes, bridges, multipolar anaphase, laggard, and c‑metaphase were also observed. Conclusion: From the results, it is evident that the percentage of MI is inversely proportional, and chromosomal aberrations (CAs) are directly proportional to the concentration and duration of exposure.

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      Daphedar, A. B., Kakkalameli, S. B., Melappa, G., Taranath, T. C., Srinivasa, C., Shivamallu, C., Syed, A., Marraiki, N., Elgorban, A. M., Veerapur, R., Patil, S. S., & Kollur, S. P. (2021). Genotoxic Assay of Silver and Zinc Oxide Nanoparticles Synthesized by Leaf Extract of Garcinia livingstonei T. Anderson: A Comparative Study. Pharmacognosy Magazine, 17(5S), S114–S121. https://doi.org/10.4103/pm.pm_536_20