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    Pharmacognosy MagazineNOW IN
    Article

    Phytochemical Screening and Antioxidant Activity of the Methanolic Extract of the Leaves of Cinnamomum tamala

    Asna Khan2,3★, Sultan Zahiruddin2★, Mohammad Ibrahim2, Parakh Basist2, Gaurav Gaurav2, Rabea Parveen2, Shahid Umar3, Sayeed Ahmad2★★ Corresponding author

    1. 1Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, India.
    2. 2Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.

    CORRESPONDENCE

    Asna Khan

    Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.

    sahmad_jh@yahoo.co.in

    Received: 20-08-2020; Revised: 23-11-2020; Accepted: 16-02-2021.

    Volume 17, Issue 5S · pp. S21–S28 · PUBLISHED 10 June 2021 · DOI: 10.4103/pm.pm_326_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: Carica papaya Linn. a member of the Caricaceae family, is a tropical fruit, rich in various secondary metabolites owing to its antioxidant, anti‑inflammatory, anti‑diabetic, and antihelmintic properties. The study was carried out to investigate the metabolic profiling and free radical scavenging compounds in C. papaya fruit’s pulp, peel and seeds through thin‑layer chromatography‑mass spectrometry (TLC‑MS) bioautography. Methodology: Aqueous, hydroalcoholic and alcoholic extracts of fruit pulp and peel were prepared along with the hydroalcoholic and hexane extract of seeds. These were subjected to total phenol, flavonoid and free radical scavenging estimation. Qualitative and quantitative high‑performance thin‑layer chromatography analysis of the best extracts was performed followed by TLC‑MS bio autography assay for the detection of free radical scavenging compounds. Results: C. papaya peel was noted to contain the highest phenol and flavonoid content, but the seeds showed better free radical scavenging activity. The hydroalcoholic extracts of pulp, peel, and seeds examined through TLC‑bioautography showed the presence of chlorogenic acid, ellagic acid, quercetin, β‑sitosterol, linoleic acid, and iso‑oleic acid as potent free radical scavenging compounds. Liquid chromatography‑mass spectrometry analysis showed the presence of β‑carotene, lycopene, and β‑cryptoxanthin in pulp and peel along with other carotenoids and benzyl isothiocyanate, linoleic acid, oleic acid, and methyl palmitate were the major compounds detected in seeds through gas chromatography‑mass spectrometry analysis. Conclusion: This study has revealed that C. papaya fruit and seeds possess potent free radical scavenging compounds. Seeds which make up the waste material may be utilized in cosmetic industries as they signify rich antioxidants.

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      Khan, A., Zahiruddin, S., Ibrahim, M., Basist, P., Gaurav, G., Parveen, R., Umar, S., & Ahmad, S. (2021). Phytochemical Screening and Antioxidant Activity of the Methanolic Extract of the Leaves of Cinnamomum tamala. Pharmacognosy Magazine, 17(5S), S21–S28. https://doi.org/10.4103/pm.pm_326_20