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    Article

    Callus Induction and Shoot Regeneration from the Immature Flower Bud of Caesalpinia bonducella and its Antileptospiral Potential by in vitro and in silico Analysis

    S. R. Santosh Kumar1, Chandrashekar Srinivasa2, Chandan Shivamallu3, Kollur Shiva Prasad4, Sushma Pradeep3, Asad Syed5, Ali Bhakali5, Amar Shankar6, Sharanagouda S Patil7, Ashwini P8, Chandan D3, Triveni R9, Govindappa Melappa10, V. Krishna11★★ Corresponding author

    1. 1Departments of Studies in Food Technology, University, Shivagangotri, Davangere.
    2. 2Studies in Biotechnology, Davangere University, Shivagangotri, Davangere.
    3. 3Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research.
    4. 4Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, Karnataka, India.
    5. 5Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
    6. 6Department of Food Technology, School of Engineering and Technology, JAIN (Deemed-to-be University).
    7. 7ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Yelahanka, Bengaluru.
    8. 8Department of Microbiology, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru.
    9. 9Sri H.N. Ananthkumar, P.U.College, Bidadi, Bengaluru.
    10. 10Department of Studies in Botany, Davangere University, Shivagangotri, Davangere.
    11. 11Department of Biotechnology, Kuvempu University, Shivamogga, Karnataka, India.

    CORRESPONDENCE

    V. Krishna

    Department of Biotechnology, Kuvempu University, Shivamogga, Karnataka, India.

    chandans@jssuni.edu.in

    Received: 10-08-2020; Revised: 04-09-2020; Accepted: 24-02-2021.

    Volume 17, Issue 5S · pp. S38–S44 · PUBLISHED 10 June 2021 · DOI: 10.4103/pm.pm_331_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The aim of the study is to establish the C. bonducella through tissue culture technique and to investigate the antileptospiral activity through computational and in vitro screening. Materials and Methods: Morphogenic calli were initiated from 96% of immature flower buds on MS medium supplemented with 2, 4 D (2, 4‑Dichlorophenoxyacetic acid) 2.0 mg/l and 6‑Benzylaminopurine (BAP) 0.5 mg/l. The calli formed were excised and subcultured on MS medium. Extreme percentage organogenesis (84%) and average shoots per culture were determined on MS medium fortified with 3.0 mg/l BAP and 0.5 mg/l indole‑3‑butyric acid (IBA). The addition of IBA in ½ MS medium favored the development of recovered shoots. Out of 30 shoots transferred to soil, 27 survived once acclimated. The isolated compounds were selected for in vitro and in silico screening. The primary pharmacological assay for leptospirosis was carried out by test tube dilution and microdilution technique and the computational screening was done using molecular docking. Results: The phytoconstituents obtained from the medicinal plant showed promising results in both in vitro and in silico antileptospiral activity. Conclusion: The LC3 and SC2 compounds isolated from C. bonducella were evaluated on Gram‑negative bacteria L. interrogans. The assay and molecular docking studies revealed the efficacy of phytocomponents of C. bonducella as traditional medicine which has an ability to cure bacterial diseases.

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      Kumar, S. R. S., Srinivasa, C., Shivamallu, C., Prasad, K. S., Pradeep, S., Syed, A., Bhakali, A., Shankar, A., Patil, S. S., P, A., D, C., R, T., Melappa, G., & Krishna, V. (2021). Callus Induction and Shoot Regeneration from the Immature Flower Bud of Caesalpinia bonducella and its Antileptospiral Potential by in vitro and in silico Analysis. Pharmacognosy Magazine, 17(5S), S38–S44. https://doi.org/10.4103/pm.pm_331_20