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    Article

    Inhibitory Roles of Nigella sativa Seed Extracts on in vitro Glycation and Aggregation

    Umme Rubab1, Dinesh Kumar1, Mohammad Abul Farah2, Khalid Mashay Al‑Anazi2, M. Ajmal Ali3, Ahmad Ali1★★ Corresponding author

    1. 1Department of Life Sciences, University of Mumbai, Mumbai, Maharashtra, India.
    2. 2Department of Zoology, College of Science, King Saud University.
    3. 3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.

    CORRESPONDENCE

    Ahmad Ali

    Department of Life Sciences, University of Mumbai, Mumbai, Maharashtra, India.

    ahmadali@mu.ac.in

    Received: 31-12-2020; Accepted: 26-07-2021.

    Volume 17, Issue 6S · pp. S220–S224 · PUBLISHED 15 September 2021 · DOI: 10.4103/pm.pm_604_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The aim of the present study was to check the effect of Nigella sativa seed extracts on glycation and aggregation and for this purpose, two proteins (bovine serum albumin [BSA] and hemoglobin) and two sugars (glucose, and ribose) were selected. Materials and Methods: The amount of glycation mediated products was measured by established methods such as browning, nitroblue tetrazolium (NBT) assay, carbonyl content, and aggregation assays in the presence or absence of extracts (1 mg/ml). The effect of extracts was also checked on glycated DNA by agarose gel electrophoresis. Results: Analysis of results indicates that extracts (1 mg/ml) significantly prevented the generation of early products up to 30.57% (BSA‑Rib) and 64.90% (Hb‑Glu). The AGEs formation was also suppressed in different glycation systems up to 60.76% (BSA‑Glu), 30.79% (BSA‑Rib) and 10.74% (Hb‑Glu) in the presence of extracts. There was also a decrease in the level of glycation‑mediated protein aggregation. Seed extracts were able to prevent the glycoxidative damage of DNA. Conclusion: It can be concluded that black cumin seeds have antiglycating and antiaggregation potential as well as antioxidant properties. Further characterization will help in the identification of active compounds which can be used for the prevention of secondary complications of diabetes and aggregation.

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      Rubab, U., Kumar, D., Farah, M. A., Al‑Anazi, K. M., Ali, M. A., & Ali, A. (2021). Inhibitory Roles of Nigella sativa Seed Extracts on in vitro Glycation and Aggregation. Pharmacognosy Magazine, 17(6S), S220–S224. https://doi.org/10.4103/pm.pm_604_20