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    Article

    Effect of Salinity Stress on Growth, Water Content, and Guggulsterone Production in Callus Cultures of Commiphora wightii (Arnott.) Bhandari

    Soumi Datta1, Jatin Katna1, Dhiraj Shrivastav1, Sarvepalli Badari Narayan1, Shahid Umar2, Jatavallabhula Lakshminarayana Sastry3, Ranjan Mitra1, Sayeed Ahmad4★★ Corresponding author

    1. 1Dabur Research and Development Centre, Ghaziabad, Uttar Pradesh.
    2. 2Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard.
    3. 3National Medicinal Plant Board (NMPB), Ministry of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homeopathy (AYUSH), Government of India.
    4. 4Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, SPER, Jamia Hamdard, New Delhi, School of Chemical and Life Sciences, Jamia Hamdard, India.

    CORRESPONDENCE

    Sayeed Ahmad

    Department of Pharmacognosy and Phytochemistry, Bioactive Natural Product Laboratory, SPER, Jamia Hamdard, New Delhi, School of Chemical and Life Sciences, Jamia Hamdard, India.

    sahmad_jh@jamiahamdard.ac.in

    Received: 27-11-2020; Revised: 29-12-2020; Accepted: 16-02-2021.

    Volume 17, Issue 6S · pp. S225–S232 · PUBLISHED 15 September 2021 · DOI: 10.4103/pm.pm_514_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: A traditional herb Commiphora wightii (Arnott.) is well known for the management of different ailments, including hypolipidemic and hypocholesterolemic disorders. Overexploitation has resulted in enlisting it under endangered species list; thus, in vitro studies are desirable to circumvent further depletion of the wild population. The objective of our study is to analyze the effect of salinity stress on growth and guggulsterone content in callus cultures. Materials and Methods: The establishment of leaf‑derived calli of C. wightii and the effect of salinity stress such as calcium chloride (CaCl2), sodium chloride (NaCl), potassium chloride, and ammonium sulfate at different concentrations and time periods on callus growth and steroid biosynthesis were studied. High‑performance liquid chromatography and liquid chromatography‑mass spectrometry/ mass spectrometry‑based spectrometric systems were carried to identify the guggulsterones. Results: The 50 mg/L CaCl2‑induced callus was found to have the highest content of guggulsterones after 15 months of incubation as compared to 9 and 12 months and indicated a positive correlation between callus age and guggulsterone biosynthesis. Conclusion: Salinity stress played an important role in callus growth of C. wightii, while calcium-induced calli showed in vitro synthesis of guggulsterones, and this can be further explored for the synthesis of guggulsterones in vitro on large commercial scale using bioreactor, and thus helps to conserve the endangered species in its natural habitat.

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      Datta, S., Katna, J., Shrivastav, D., Narayan, S. B., Umar, S., Sastry, J. L., Mitra, R., & Ahmad, S. (2021). Effect of Salinity Stress on Growth, Water Content, and Guggulsterone Production in Callus Cultures of Commiphora wightii (Arnott.) Bhandari. Pharmacognosy Magazine, 17(6S), S225–S232. https://doi.org/10.4103/pm.pm_514_20