Ethacridine Regulation of JAK/STAT/ERK Signaling Pathway in Colon Cancer Cells SW620: In vitro Approach
Hui Zhang1, Hui Yang2, Wei Wei3, Jing Zhao2, Annamalai Vijayalakshmi4, Minhong Wang5★★ Corresponding author
- 1Department of Gastroenterology, Hanzhong Central Hospital, Hanzhong, CHINA.
- 2Department of Oncology Rehabilitation, Shaanxi Kangfu Hospital, Xi’an, CHINA.
- 3Department of Pathology, Xi’an No. 3 Hospital, Xi’an, CHINA.
- 4Department of Biochemistry, Rabiammal Ahamed Maideen College for Women, Thiruvarur, Tamil Nadu, INDIA.
- 5Department of Digestive Oncology, Pucheng County Hospital, Weinan, CHINA.
CORRESPONDENCE
Minhong Wang
Department of Digestive Oncology, Pucheng County Hospital, Weinan 715500, CHINA.
Received: 15-01-2022; Accepted: 01-06-2022.
Volume 19, Issue 2 · pp. 214–221 · PUBLISHED 2023 · DOI: 10.1177/09731296221136906
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ABSTRACT
Background: Ethacridine have anticancer effects by inhibiting regulatory transcription factors and cell viability in various cancer cells. To investigate the effect of ethacridine on colorectal cancer cell lines, SW620 was studied via regulation of JAK/STAT/ERK signaling pathway. Materials and Methods: Different doses of ethacridine (5–35 μM) expressed antiproliferative effects by decreasing the viability in a dose-dependent manner and the IC50 value was found to be 10 μM. Results: Subsequent treatment with 10 μM of ethacridine showed that it induced mitochondrial dysfunction and reactive oxygen species generation. DAPI and PI staining assays revealed prominent apoptotic cells under the microscope when treated with 10 μM of ethacridine. In the mRNA expression study performing RT-PCR of apoptotic markers, cyclin-D1, Bax, Bcl-2, caspase 3, C-Myc, and surviving, enhanced levels of these markers were suppressed, which was inversely proportional to the levels of apoptotic enhancers namely Bax and caspase-3. It was also observed that increased NF-κB, IL-6, TNF-α, and COX-2 in colorectal cancer are suppressed by ethacridine. The expressions of JAK/STAT/ERK were also significantly suppressed after ethacridine treatment in SW620 cell lines. Conclusion: In summary, it was corroborated that ethacridine promoted apoptosis in colon cancer cells by inhibiting quite a few cell signaling factors.
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Zhang, H., Yang, H., Wei, W., Zhao, J., Vijayalakshmi, A., & Wang, M. (2023). Ethacridine Regulation of JAK/STAT/ERK Signaling Pathway in Colon Cancer Cells SW620: In vitro Approach. Pharmacognosy Magazine, 19(2), 214–221. https://doi.org/10.1177/09731296221136906