LC-MS/MS-based Targeted Metabolomic Profiling of Aqueous and Hydro-alcoholic Extracts of Pistacia integerrima Linn., Quercus infectoria Olivier and Terminalia chebula Retz
Anil Kumar Saini1, Laxman Sawant1, Sultan Zahiruddin2, Dhiraj Shrivastva1, Ranjan Mitra1, Rajiva Kumar Rai1, Sayeed Ahmad2★★ Corresponding author
- 1Dabur Research and Development Centre, Dabur India Limited, Sahibabad, Ghaziabad, Uttar Pradesh, INDIA.
- 2Bioactive Natural Product Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, INDIA.
CORRESPONDENCE
Sayeed Ahmad
Bioactive Natural Product Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, INDIA.
Volume 19, Issue 2 · pp. 222–230 · PUBLISHED 2023 · DOI: 10.1177/09731296221144809
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ABSTRACT
Background: Pistacia integerrima (PI) galls, being rich in polyphenolic compounds, owing to its high restorative worth and restricted assets and exorbitant utilization lead to the overexploitation of the species and availability issues. It is expected to categorise under the red list of threatened taxa. Ayurveda text Bhaishajaya Ratnavali mentioned Quercus infectoria (QI) as ‘pratinidhi’ or substitute of PI and Yogaratnakara (abhavvarga) text reference indicated Terminalia chebula (TC) having the same therapeutic character as in PI. Hence, this study was focused on the comparative quantification of major polyphenolic compounds in aqueous and hydro-alcoholic extracts of PI, QI and TC by liquid chromatography and tandem mass spectroscopy (LC‑MS/MS). Objectives: The LC‑MS/MS method was used for the determination of polyphenolic compounds in aqueous and hydroalcoholic extracts of PI, QI and TC. Materials and Methods: The LC-MS/MS method was developed for the quantification of targeted polyphenolic compounds and validated in terms of linearity, accuracy, precision, limit of detection and limit of quantification. Analysis performed by LC-MS/MS 6460 C triple quad coupled with Agilent HPLC. The chromatographic separation was performed on the RP C18 column with gradient elution. Results: The developed method of LC-MS/MS was used for the quantitative estimation of gallic acid, corilagin acid, caffeic acid, syringic acid, rutin hydrate, ellagic acid, quercetin and morin hydrate in PI, QI and TC. As per method validation parameters, calibration plots were found to be linear, accurate, precise and specific for LC-MS/MS. The calibration plot exhibited an excellent coefficient of determination (R2 ≥ 0.99), and extraction recovery was found 96.20%–103.71%. Gallic acid and ellagic acid are the most abundant compound among eight targeted polyphenolics in all extracts. Conclusion: The finding of LC-MS/MS analysis of polyphenolic compounds in aqueous and hydro-alcoholic extracts indicates that PI, QI and TC are having phytochemical similarities up to an extent in polyphenolic compounds as a secondary metabolite; however, a firmer opinion can be formed after a comparative efficacy study and the evaluation of all three extracts.
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Saini, A. K., Sawant, L., Zahiruddin, S., Shrivastva, D., Mitra, R., Rai, R. K., & Ahmad, S. (2023). LC-MS/MS-based Targeted Metabolomic Profiling of Aqueous and Hydro-alcoholic Extracts of Pistacia integerrima Linn., Quercus infectoria Olivier and Terminalia chebula Retz. Pharmacognosy Magazine, 19(2), 222–230. https://doi.org/10.1177/09731296221144809