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    Network Pharmacology and Experimental Analyses on Astragalus membranaceus (Huangqi) Effects on Endotoxin-induced Uveitis Model in Rats

    Jing Lu1,3, Yujie Wu1, Yijing Yang2,4, Jing Zhou6, Bo Huang2, Chengfeng Xie1, Qinghua Peng4,5★, Xiaohuan Zhang1★ Corresponding author

    1. 1Medical School, Hunan University of Chinese Medicine, Changsha, Hunan, People’s Republic of China.
    2. 2Chinese Medical School, Hunan University of Chinese Medicine, Changsha, Hunan, People’s Republic of China.
    3. 3Hunan Provincial Engineering Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Hunan, People’s Republic of China.
    4. 4Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan, People’s Republic of China.
    5. 5Hunan Provincial Engineering Research Center for Prevention and Treatment of Ophthalmology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, Hunan, People’s Republic of China.
    6. 6Imaging Center, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, People’s Republic of China.

    CORRESPONDENCE

    Qinghua Peng

    Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, People’s Republic of China.

    pqh410007@126.com

    Received: 14-04-2022; Accepted: 26-10-2022.

    Volume 19, Issue 3 · pp. 751–762 · PUBLISHED 2023 · DOI: 10.1177/09731296231169576

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study is aimed at identifying critical therapeutic targets of Astragalus membranaceus (Huangqi (HQ)) and investigating the effects and mechanisms of HQ treating uveitis. The potential drug targets of HQ and main active ingredients were obtained from the Traditional Chinese medicine (TCM) systems pharmacology database and analysis platform (TCMSP, http://tcmspnw.com). Materials and Methods: Cytoscape software was used to identify the disease targets of uveitis. Drug targets and disease targets were compared, and intersected hubs were applied for the active ingredient-target network and protein-proteininteraction (PPI) network construction. Signaling pathway enrichment annotation was performed to identify possible signaling involved in uveitis treatment. An endotoxin-induced uveitis (EIU) model was established, and the therapeutic effects of total flavonoids of Astragalus (TFA) on uveitis were investigated by examining the improvement of eye symptoms, histopathological alterations, and the levels of cytokines. Results: Based on network pharmacological analysis, HQ could modulate the initiation and progression of uveitis by reducing the production of cytokines and regulating cell apoptosis via the NOD-like receptor (NLR), apoptosis, and toll-like receptor (TLR) signaling pathways. Based on animal experiments, high-dose TFA could reduce rat’s iris congestion, reduce anterior chamber exudation and pus, restore pupil size, and decrease the release of inflammatory factors IFN-γ and IL-10. Network pharmacological and experimental analyses revealed that TFA regulates the release of inflammatory factors through the NLR and TLR signaling pathways, thus regulating the immune system of EIU rats and ultimately relieving inflammation responses in uveitis rats.

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      Lu, J., Wu, Y., Yang, Y., Zhou, J., Huang, B., Xie, C., Peng, Q., & Zhang, X. (2023). Network Pharmacology and Experimental Analyses on Astragalus membranaceus (Huangqi) Effects on Endotoxin-induced Uveitis Model in Rats. Pharmacognosy Magazine, 19(3), 751–762. https://doi.org/10.1177/09731296231169576