RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
Liying Zhang1, Ting Zhou1, Yiming Zhang1, Lei Wang2, Yangyang Li1, Gucheng Zhou1, Heng Zhou3, Fan Niu4, Zhiwei Liu1★, Zhiming Miao1, Yongqi Liu1,5★ Corresponding author
- 1Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, China.
- 2School of Public Health, Yangzhou University, Yangzhou, CHINA.
- 3Chinese Academy of Chinese Medical Sciences, Beijing, CHINA.
- 4Provincial Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, CHINA.
- 5Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Gansu University of Chinese Medicine, Lanzhou, CHINA.
CORRESPONDENCE
Zhiwei Liu
Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, China Corresponding author: Yongqi Liu, Provincial Level Key Laboratory for Molecular Medicine of Major Diseases and Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine, Lanzhou, China; Key Laboratory of Dunhuang Medicine and Transformation at Provincial and Ministerial Level, Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, China. . Email: liuyongqi73@163.com
Received: 28-12-2022; Accepted: 02-07-2023.
Volume 20, Issue 2 · pp. 516–527 · PUBLISHED 2024 · DOI: 10.1177/09731296231204762
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ABSTRACT
Background: Ionizing radiation can induce bystander effects (RIBE), and Astragalus polysaccharides (APS) have a protective effect against RIBE. It has been proved that RIBE occurred and APS played the inhibitory effects on the RIBE when radiation was carried out only one time. However, whether RIBE happened by multiple irradiations and the effects of APS on the RIBE are unclear. This study aimed to investigate whether APS suppress RIBE damage induced by multiple irradiations. Materials and Methods: A549 cells were irradiated with 2 Gy X-rays to obtain a conditioned medium. Bone mesenchymal stem cells (BMSCs) was incubated with the conditioned medium 5 times every 3 days or APS. Cell proliferation was detected by CCK-8 and colony formation assay, and genomic instability and DNA damages were detected by the micronucleus and immunofluorescence assay. The protein associated with methylation and cancer-associated proteins in BMSCs were detected by western blot. Results: After five stimulations with a conditioned medium, the proliferative capacity of BMSCs decreased; APS preintervention promoted the proliferation of BMSCs. And conditional medium intervention increased the micronucleus rate and a number of 53BP1 foci; APS pre-intervention reduced the micronucleus rate and number of 53BP1 foci. In addition, the conditional medium intervention reduced the expression of methylation-related proteins and increased the expression of cancer-related proteins, while APS pre-intervention reversed this trend. Conclusion: Irradiated A549 conditioned medium can induce RIBE of BMSCs, which might be related to methylation and cancer-associated proteins and APS may block RIBE by multiple irradiations in BMSCs.
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Zhang, L., Zhou, T., Zhang, Y., Wang, L., Li, Y., Zhou, G., Zhou, H., Niu, F., Liu, Z., Miao, Z., & Liu, Y. (2024). RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs. Pharmacognosy Magazine, 20(2), 516–527. https://doi.org/10.1177/09731296231204762