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    Article

    Study on the Fingerprints of Acanthopanax trifoliatus Fractions with Varying Polarities and the Relationship Between Anti-inflammatory and Antioxidant Activity Using Multivariate Analysis

    Zijiu Liang1, Xiaohong Huang1, Lin Jiang1,2,3, Qinghua Lin1,3, Renyi Zhang1, Changyuan Lin1, Xinghong Lai1, Mengjiao Xie1, Haiyan Chen1, Qing Chen3, Fangchan Li1,2,3★★ Corresponding author

    1. 1Department of Chinese Materia Medica, College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, CHINA.
    2. 2Department of Key Laboratory of TCM Extraction and Purification and Quality Analysis, Teaching Experiment Training Center, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, CHINA.
    3. 3Department of Pharmaceutical Engineering Technology Research Center of Guangxi Zhuang and Yao, Science Experiment Center, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, China.

    CORRESPONDENCE

    Fangchan Li

    Department of Chinese Materia Medica, College of Pharmacy, Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, CHINA.

    1936490308@qq.com

    Received: 03-02-2023; Accepted: 18-08-2023.

    Volume 20, Issue 2 · pp. 528–540 · PUBLISHED 2024 · DOI: 10.1177/09731296231201032

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: To evaluate the Spectrum-Effect relationships between the High-Performance Liquid Chromatography (HPLC) Fingerprints of fractions with different polarities and the anti-inflammatory and antioxidant effects of A. trifoliatus and to identify its pharmacodynamic material basis. Materials and Methods: Chemical Fingerprints of A. trifoliatus fractions with different polarities (all fractions and ethyl acetate, n-butanol and water fractions) were obtained by HPLC, and Principal Component Analysis (PCA) was performed. The foot swelling and prostaglandin E2 (PGE2) content in mice induced by carrageenan were used as anti-inflammatory markers, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods were used as antioxidant indicators. Moreover, the Spectrum-Effect relationship between common peaks and efficacy value was conducted by Grey Relational Analysis (GRA) and Partial Least Squares analysis (PLS). Results: PCA identified three principal components, and all fractions of A. trifoliatus had good anti-inflammatory and antioxidant effects. Based on GRA and PLS analysis, peaks 1 and 2 (neochlorogenic acid), 4 and 5 (chlorogenic acid), 8, 9, 10, 11 (isochlorogenic acid B), and 12 were involved in anti-inflammatory efficacy, and peaks 1 and 2 (neochlorogenic acid), 3, 4, and 5 (chlorogenic acid), and 6, 7, 9, 10, and 13 (isochlorogenic acid A) were involved in antioxidant efficacy. Conclusion: This study on the Spectrum-Effect relationship of different polar fractions of A. trifoliatus provides data to support the discovery of the pharmacodynamic material basis of the anti-inflammatory and antioxidant properties of A. trifoliatus.

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      Liang, Z., Huang, X., Jiang, L., Lin, Q., Zhang, R., Lin, C., Lai, X., Xie, M., Chen, H., Chen, Q., & Li, F. (2024). Study on the Fingerprints of Acanthopanax trifoliatus Fractions with Varying Polarities and the Relationship Between Anti-inflammatory and Antioxidant Activity Using Multivariate Analysis. Pharmacognosy Magazine, 20(2), 528–540. https://doi.org/10.1177/09731296231201032