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    Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line

    Mahnaz Khanavi1★, Razieh Gheidarloo1, Nargess Sadati1, Mohammad Reza Shams Ardekani1, Seyed Mohammad Bagher Nabavi2, Shohreh Tavajohi3, Seyed Nasser Ostad3★ Corresponding author

    1. 1Departments of Pharmacognosy and Medicinal Plants Research Center, University of Medical Sciences, Tehran 14155-6451, Iran.
    2. 2Khorramshahr University of Marine Science and Technology, Khuzestan, Iran.
    3. 3Toxicology and Pharmacology and Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran 14155-6451, Iran.

    CORRESPONDENCE

    Mahnaz Khanavi

    Departments of Pharmacognosy and Medicinal Plants Research Center, University of Medical Sciences, Tehran 14155-6451, Iran.

    Received: 01-06-2011; Revised: 16-07-2011.

    Volume 8, Issue 29 · pp. 60–64 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93327

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Context: Marine algae produce different secondary metabolites with a wide range of biological activities. Many studies have been achieved on the screening of biological effects of marine organisms and a lot of active compounds were isolated and characterized. Aims: In an attempt to fi nd cytotoxic compound of hexane fraction, isolation, identifi cation, and cytotoxicity of active compound of this fraction were performed. Materials and Methods: In this study, total methanolic (70%) extract and partition fractions of hexane, chloroform (CHCl3), ethyl acetate (EtOAc), and MeOH–H2O of Sargassum angustifolium, Chondria dasyphylla, and Ulva fl exuosa, collected from coastlines of the Persian Gulf in south of Iran, were studied against colon carcinoma (HT-29), colorectal adenocarcinoma (Caco-2), breast ductal carcinoma (T47D), and Swiss mouse embryo fi broblast (NIH 3T3) cell lines by MTT assay. Statistical Analysis Used: IC50 (median growth inhibitory concentration) values were calculated by Sigmaplot (10) software. Results: Hexane fraction of Chondria dasyphylla (IC50 82.26 ± 4.09 μg/ml) and MeOH-H2O fraction of Ulva fl exuosa (IC50 116.92 ± 8.58 μg/ml) showed cytotoxic activity against proliferation of T47D cells. Hexane fraction of Sargassum angustifolium was also observed for cytotoxicity against T47D and HT-29 cell lines (IC50 166.42 ± 26.7 and 190.24 ± 52.8 μg/ml), respectively. An investigation of a component from the hexane fraction of Sargassum angustifolium yielded a steroidal metabolite, fucosterol, with cytotoxicity in T47D and HT29 (IC50 27.94 ± 9.3 and 70.41 ± 7.5 μg/ml). Conclusions: These results indicated that fucosterol, the most abundant phytosterol in brown algae, is responsible for cytotoxic effect of this extract against breast and colon carcinoma cell lines.

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      Khanavi, M., Gheidarloo, R., Sadati, N., Ardekani, M. R. S., Nabavi, S. M. B., Tavajohi, S., & Ostad, S. N. (2012). Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line. Pharmacognosy Magazine, 8(29), 60–64. https://doi.org/10.4103/0973-1296.93327