Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Anti-hepatotoxicity Effect of Orthosiphon stamineus Benth against Acetaminophen-induced Liver Injury in Rats by Enhancing Hepatic GST Activity

    J.H. Chin1★, A.H. Hussin2, S. Ismail3★ Corresponding author

    1. 1School of Pharmacy, Faculty of Medical Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, 56000. Kuala Lumpur, Malaysia.
    2. 2School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800. Minden, Penang, Malaysia.
    3. 3Centre for Drug Research, Universiti Sains Malaysia, 11800. Minden, Penang, Malaysia.

    CORRESPONDENCE

    J.H. Chin

    *Author of Correspondence: Dr. Chin Jin Han or jhchin@ucsi.edu.my Tel: +603-91018880 ext 3072; Fax: +603-91023606

    jinhanchin@hotmail.com

    Received: 02-02-2009; Accepted: 26-02-2009.

    Volume 1, Issue 2 · pp. 53–58 · PUBLISHED Mar-Apr 2009 · DOI:

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    The present study was undertaken to assess the sub-chronic (14-day treatment) effect of oral administration of O. stamineus on hepatic drug metabolising enzymes and the protective effect against acetaminophen-induced liver injury in Sprague Dawley (SD) rats. Healthy male SD rats weighing 200 g ± 10 g were used throughout the experiments. Isolated hepatocytes and liver cytosolic fraction were used to examine the effect of O. stamineus on the activity of aminopyrine N-demethylase and glutathione-S-transferase respectively. Hepatoprotective effect of O. stamineus was judged by comparing the serum levels of hepatic markers, i.e. AST, ALT and ALP and the percentage of hepatocytes viability between O. stamineus treatment groups and acetaminophen (APAP) control group. O. stamineus extract at 125 mg/kg (P<0.01) and 500 mg/kg (P<0.01) exhibited significant hepatoprotective effect by restoring the elevation of serum levels of AST and ALT and increased the percentage of hepatocytes viability in rats. The hepatoprotective effect exhibited by O. stamineus at dose 500 mg/kg was comparable to silymarin at dose 20 mg/kg in acetaminophen-induced liver injury rats. The activity of GST was 17% higher in the rats treated with 500 mg/kg (P<0.01) of O. stamineus compared with the control group. There was no significant difference in body weigh gained, food consumption, water intake and relative organ weight between the treatment group and the control group. Methanol extract of O. stamineus protects against acetaminophen-induced liver injury in rats by enhancing the activity of GST in liver.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Chin, J., Hussin, A., & Ismail, S. (2009). Anti-hepatotoxicity Effect of Orthosiphon stamineus Benth against Acetaminophen-induced Liver Injury in Rats by Enhancing Hepatic GST Activity. Pharmacognosy Research, 1(2), 53–58.