Effect of methyl jasmonate on production of ariltetralin lignans in hairy root cultures of Linum tauricum
Iliana Ionkova1★★ Corresponding author
- 1Faculty of Pharmacy, Medical University of Sofia, Dunav Str. 2, 1000 Sofia, Bulgaria
Received: 19-03-2009; Accepted: 09-04-2009.
Volume 1, Issue 3 · pp. 102–105 · PUBLISHED May-Jun 2009 · DOI:
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ABSTRACT
Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ariltetraline lignans, which are considered to be the main active compounds in Linum tauricum. This study was concentrated on the induction of production of ariltetralin lignans in hairy roots cultures of L. tauricum, transformed by Agrobacterium rhyzogenes, ATCC 15834 by exposing them to different concentrations (50-200 microM) of methyl jasmonate (MeJA) during the culture period. The content of 4’-demethyl-6-methoxypodo-phylotoxin (4’-DM- 6MPTOX) and 6-methoxypodophyllotoxin (MPTOX), the main constituents in hairy roots of L. tauricum increased about 1.2 folds by elicitation of MeJA, however, the fresh weight, dry weight and growth ratio was inhibited by increasing MeJA concentrations. The highest total lignans yield was obtained with 150 microM MeJA treatment. These results suggest that MeJA elicitation is beneficial for lignan accumulation in the hairy roots cultures of L. tauricum.
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Ionkova, I. (2009). Effect of methyl jasmonate on production of ariltetralin lignans in hairy root cultures of Linum tauricum. Pharmacognosy Research, 1(3), 102–105.